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PMID: 2461450 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Immunological characterization of rat cardiac gap junctions: presence of common antigenic determinants in heart of other vertebrate species and in various organs.

The Journal of membrane biology ·Vol. 104 ·No. 2 ·1988-09-00 ·Pages 119-28

Dupont E, el Aoumari A, Roustiau-Sévère S, Briand JP, Gros D

Abstract

Antibodies to the following synthetic peptide, SALGKLLDKVQAY, were purified by affinity chromatography and characterized by ELISA and immunoblotting. These antibodies, shown to be specific to the major protein constituent of isolated rat heart junctions: connexin 43, cross-reacted with a homologous protein in immunoreplicas of whole heart fractions of trout, frog, chicken, guinea pig, mouse and rat, suggesting a phylogenic conservation of connexin 43 in vertebrates. By immunoblotting of whole organ fractions it was also demonstrated that these antibodies cross-reacted with major proteins of Mr 32 and 22 kD in rat and mouse liver, of Mr 41 kD in rat cerebellum, of Mr 43 kD in uterus, stomach and kidney of rat, of Mr 46 and 70 kD in rat lens, suggesting that these proteins share common or related epitopes with the synthetic peptide and connexin 43.

MeSH Terms
Amino Acid Sequence Animals Chickens/immunology Connexins Cross Reactions Electrophoresis, Polyacrylamide Gel Enzyme-Linked Immunosorbent Assay Epitopes/analysis,immunology Female Fishes/immunology Guinea Pigs Immunoblotting Immunoglobulin G/immunology,isolation & purification Intercellular Junctions/immunology Membrane Proteins/analysis,immunology Mice Molecular Sequence Data Myocardium/analysis,immunology,ultrastructure Organ Specificity Peptide Fragments/chemical synthesis,immunology Ranidae/immunology Rats Species Specificity
Chemicals
Connexins Epitopes Immunoglobulin G Membrane Proteins Peptide Fragments
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Dupont E
Laboratory of Cellular Differentiation Biology, CNRS 179, Faculty of the Sciences of Luminy, Marseille, France.
el Aoumari A
Roustiau-Sévère S
Briand J P
Gros D
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Article Info
Journal
The Journal of membrane biology
Abbr.
J Membr Biol
ISSN
0022-2631
DOI
10.1007/BF01870924
Published
1988-09-00
Pages
119-28
Language
English
Region
United States
NLM ID
0211301
Subset
IM
External Links
PubMed source DOI: 10.1007/BF01870924 View journal details
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