Home LiteratureArticle Details
PMID: 3701846 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Rat heart gap junctions as disulfide-bonded connexon multimers: their depolymerization and solubilization in deoxycholate.

The Journal of membrane biology ·Vol. 90 ·No. 1 ·1986-00-00 ·Pages 43-57

Manjunath CK, Page E

Abstract

Unproteolyzed gap junctions isolated from rat heart and liver were analyzed for the presence of inter-subunit disulfide bonds by sodium dodecylsulfate polyacrylamide gel electrophoresis. Rat cardiac junctions contained multiple disulfide bonds connecting the Mr 47,000 subunits of the same connexon and of different connexons. Inter-subunit disulfide bonds were absent in liver junctions. Unproteolyzed rat heart gap junctions were resistant to deoxycholate in their oxidized state, but dissolved readily in the detergent when the disulfide bonds were cleaved with beta-mercaptoethanol. Disulfide bonding in proteolyzed cardiac junctions was limited to pairs of Mr 29,500 subunits. These junctions were not soluble in deoxycholate even in the presence of beta-mercaptoethanol. These results show that heart and liver junctions differ in their quarternary organization.

MeSH Terms
Animals Cell Fractionation Deoxycholic Acid/pharmacology Disulfides/analysis Electrophoresis, Polyacrylamide Gel Heart/drug effects Intercellular Junctions/drug effects,ultrastructure Liver/ultrastructure Membrane Proteins/analysis Microscopy, Electron Molecular Weight Myocardium/ultrastructure Rats Rats, Inbred Strains Solubility
Chemicals
Disulfides Membrane Proteins Deoxycholic Acid
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Manjunath C K
Page E
References (35)
35 references, click to expand
  1. A modification of the Lowry procedure to simplify protein determination in membrane and lipoprotein samples.
    Anal Biochem. 1978 Jun 15;87(1):206-10 PMID: 98070
  2. Hormonal regulation of cell junction permeability: upregulation by catecholamine and prostaglandin E1.
    J Membr Biol. 1982;70(3):239-51 PMID: 6313926
  3. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
    Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
  4. Isolation and protein composition of gap junctions from rabbit hearts.
    Biochem J. 1982 Jul 1;205(1):189-94 PMID: 7126176
  5. Junctional intercellular communication: the cell-to-cell membrane channel.
    Physiol Rev. 1981 Oct;61(4):829-913 PMID: 6270711
  6. Lens gap junctions: a structural hypothesis for nonregulated low-resistance intercellular pathways.
    Invest Ophthalmol Vis Sci. 1979 Nov;18(11):1104-22 PMID: 511455
  7. Effect of intracellular injection of cAMP on the electrical coupling of mammalian cardiac cells.
    Biochem Biophys Res Commun. 1984 Mar 30;119(3):1001-7 PMID: 6324775
  8. Bulk isolation of mouse hepatocyte gap junctions. Characterization of the principal protein, connexin.
    J Cell Biol. 1974 May;61(2):557-63 PMID: 4363961
  9. Lens membranes II. Isolation and characterization of the main intrinsic polypeptide (MIP) of bovine lens fiber membranes.
    Exp Eye Res. 1976 Sep;23(3):365-71 PMID: 976377
  10. Cell junction and cycle AMP: III. Promotion of junctional membrane permeability and junctional membrane particles in a junction-deficient cell type.
    J Membr Biol. 1981;63(1-2):133-46 PMID: 6273568
  11. Differences between cytoplasmic surfaces of deep-etched heart and liver gap junctions.
    Am J Physiol. 1985 Sep;249(3 Pt 2):H690-3 PMID: 4037114
  12. Correction of cell-cell communication defect by introduction of a protein kinase into mutant cells.
    Nature. 1983 Sep 29-Oct 5;305(5933):433-5 PMID: 6621694
  13. Biochemical and immunological approaches to the study of gap junctional communication.
    In Vitro. 1980 Dec;16(12):1057-67 PMID: 6163693
  14. Antibody probes in the study of gap junctional communication.
    Annu Rev Physiol. 1985;47:305-18 PMID: 2986533
  15. Lens cell-to-cell channel protein: I. Self-assembly into liposomes and permeability regulation by calmodulin.
    J Membr Biol. 1985;83(3):217-25 PMID: 3999121
  16. Biological disulfides: the third messenger? Modulation of phosphofructokinase activity by thiol/disulfide exchange.
    J Biol Chem. 1982 Oct 25;257(20):12086-91 PMID: 6214556
  17. Proteolysis of cardiac gap junctions during their isolation from rat hearts.
    J Membr Biol. 1985;85(2):159-68 PMID: 4009696
  18. A protein homologous to the 27,000 dalton liver gap junction protein is present in a wide variety of species and tissues.
    Cell. 1984 Nov;39(1):61-9 PMID: 6091916
  19. The Mr 28,000 gap junction proteins from rat heart and liver are different but related.
    J Biol Chem. 1985 Jun 10;260(11):6514-7 PMID: 2987225
  20. Detergent sensitivity and splitting of isolated liver gap junctions.
    J Membr Biol. 1984;78(2):147-55 PMID: 6425504
  21. Rapid visualization of protein bands in preparative SDS-polyacrylamide gels.
    Anal Biochem. 1979 Mar;93(2):257-60 PMID: 464259
  22. Cell junction and cyclic AMP: II. Modulations of junctional membrane permeability, dependent on serum and cell density.
    J Membr Biol. 1981;63(1-2):123-31 PMID: 6273567
  23. The glutathione status of cells.
    Int Rev Cytol. 1978;54:109-60 PMID: 42630
  24. Structure and biochemistry of mouse hepatic gap junctions.
    J Mol Biol. 1979 Aug 5;132(2):193-218 PMID: 537050
  25. Gap junction structures. II. Analysis of the x-ray diffraction data.
    J Cell Biol. 1977 Aug;74(2):629-45 PMID: 889612
  26. Cell biology and protein composition of cardiac gap junctions.
    Am J Physiol. 1985 Jun;248(6 Pt 2):H783-91 PMID: 2408491
  27. Effects of thyroxin on ultrastructure of rat myocardial cells: a stereological study.
    J Ultrastruct Res. 1973 Jan;42(1):136-55 PMID: 4567405
  28. Reduction of gap junctional conductance by microinjection of antibodies against the 27-kDa liver gap junction polypeptide.
    Proc Natl Acad Sci U S A. 1985 Apr;82(8):2412-6 PMID: 2986116
  29. Isolation and characterization of gap junctions from rat liver.
    J Biol Chem. 1979 Mar 25;254(6):2138-47 PMID: 422573
  30. Role of cytoplasmic thioltransferase in cellular regulation by thiol-disulphide interchange.
    Biochem J. 1980 Jul 15;190(1):125-30 PMID: 7447929
  31. Rat liver gap junction protein: properties and partial sequence.
    Proc Natl Acad Sci U S A. 1981 Dec;78(12):7594-8 PMID: 6278482
  32. Cytoplasmic surface and intramembrane components of rat heart gap junctional proteins.
    Am J Physiol. 1984 Jun;246(6 Pt 2):H865-75 PMID: 6430100
  33. Immunocytochemical localization of the lens main intrinsic polypeptide (MIP26) in communicating junctions.
    J Cell Biol. 1982 Jan;92(1):213-20 PMID: 7035467
  34. Evidence for intrinsic proteolytic activity in rat liver plasma membranes.
    Biochem Biophys Res Commun. 1982 Sep 30;108(2):486-94 PMID: 6756400
  35. Protein mixed-disulfides in cardiac cells. S-thiolation of soluble proteins in response to diamide.
    Biochim Biophys Acta. 1985 Jan 18;844(1):50-4 PMID: 3967051
Article Info
Journal
The Journal of membrane biology
Abbr.
J Membr Biol
ISSN
0022-2631
DOI
10.1007/BF01869685
Published
1986-00-00
Pages
43-57
Language
English
Region
United States
NLM ID
0211301
Subset
IM
Grants
NHLBI NIH HHS · HL 10503 · United States
NHLBI NIH HHS · HL 20592 · United States
External Links
PubMed source DOI: 10.1007/BF01869685 View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2