CD19, the earliest differentiation antigen of the B cell lineage, bears three extracellular immunoglobulin-like domains and an Epstein-Barr virus-related cytoplasmic tail.

The Journal of experimental medicine ·Vol. 168 ·No. 3 ·1988-09-01 ·Pages 1205-10

Stamenkovic I, Seed B

Abstract

The isolation and expression of a full-length cDNA clone encoding the B cell-specific glycoprotein CD19 is reported. The sequence of the cDNA predicts a glycosylated integral membrane protein with a precursor molecular weight of 51.8 x 10(3) and an extracellular domain organized into three contiguous Ig-like sub-domains. The cytoplasmic domain bears significant relatedness to two proteins encoded by the Epstein-Barr virus and the int-1 oncogene. CD19 transcripts are restricted to members of the B cell lineage, being most abundant in pre-B cell lines and least abundant in plasmacytomas.

MeSH Terms

Amino Acid Sequence Antigens, CD19 Antigens, Differentiation, B-Lymphocyte/genetics Base Sequence Blotting, Northern Cloning, Molecular DNA/genetics Humans Membrane Glycoproteins/genetics Molecular Sequence Data Molecular Structure

Chemicals

Antigens, CD19 Antigens, Differentiation, B-Lymphocyte Membrane Glycoproteins DNA

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Stamenkovic I

Department of Genetics, Harvard Medical School, Boston, Massachusetts.

Seed B

References (14)

14 references, click to expand

Compilation and analysis of sequences upstream from the translational start site in eukaryotic mRNAs.
Nucleic Acids Res. 1984 Jan 25;12(2):857-72 PMID: 6694911
DNA sequence and expression of the B95-8 Epstein-Barr virus genome.
Nature. 1984 Jul 19-25;310(5974):207-11 PMID: 6087149
Two major outer envelope glycoproteins of Epstein-Barr virus are encoded by the same gene.
J Virol. 1985 Jun;54(3):665-74 PMID: 2987520
The nucleotide sequence of the human int-1 mammary oncogene; evolutionary conservation of coding and non-coding sequences.
EMBO J. 1985 Nov;4(11):2905-9 PMID: 2998762
A new method for predicting signal sequence cleavage sites.
Nucleic Acids Res. 1986 Jun 11;14(11):4683-90 PMID: 3714490
CD19 monoclonal antibody HD37 inhibits anti-immunoglobulin-induced B cell activation and proliferation.
J Immunol. 1987 May 1;138(9):2793-9 PMID: 2437199
B4, a human B lymphocyte-associated antigen expressed on normal, mitogen-activated, and malignant B lymphocytes.
J Immunol. 1983 Jul;131(1):244-50 PMID: 6408173
A cDNA presumptively coding for the alpha subunit of the receptor with high affinity for immunoglobulin E.
Biochemistry. 1987 Jul 28;26(15):4605-10 PMID: 2959318
Molecular cloning of a CD28 cDNA by a high-efficiency COS cell expression system.
Proc Natl Acad Sci U S A. 1987 Dec;84(23):8573-7 PMID: 2825196
Detailed studies on expression and function of CD19 surface determinant by using B43 monoclonal antibody and the clinical potential of anti-CD19 immunotoxins.
Blood. 1988 Jan;71(1):13-29 PMID: 3257143
B cell differentiation antigens as probes for functional B cell subsets.
Immunol Rev. 1982;64:57-79 PMID: 6806173
Surface antigen expression and immunoglobulin gene rearrangement during mouse pre-B cell development.
Immunol Rev. 1982;69:5-23 PMID: 6984001
Establishing homologies in protein sequences.
Methods Enzymol. 1983;91:524-45 PMID: 6855599
Molecular cloning of the CD2 antigen, the T-cell erythrocyte receptor, by a rapid immunoselection procedure.
Proc Natl Acad Sci U S A. 1987 May;84(10):3365-9 PMID: 2437578

Article Info

Journal

The Journal of experimental medicine

Abbr.

J Exp Med

ISSN

0022-1007

DOI

10.1084/jem.168.3.1205

Published

1988-09-01

Pages

1205-10

Language

English

Region

United States

NLM ID

2985109R

PMCID

PMC2189043

Subset

Databases

GENBANK

M21097

External Links

PubMed source DOI: 10.1084/jem.168.3.1205 PMC Full Text View journal details

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CD19, the earliest differentiation antigen of the B cell lineage, bears three extracellular immunoglobulin-like domains and an Epstein-Barr virus-related cytoplasmic tail.

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