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PMID: 2457919 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Genetics of protein I of Neisseria gonorrhoeae: construction of hybrid porins.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 85 ·No. 18 ·1988-09-00 ·Pages 6841-5

Carbonetti NH, Simnad VI, Seifert HS, So M, Sparling PF

Abstract

Protein I (PI), the major outer membrane protein of Neisseria gonorrhoeae, is a porin and occurs in two major immunochemical classes, A and B. By using shuttle mutagenesis to insert a selectable marker close to the PI structural gene, evidence was obtained from transformation experiments to demonstrate that the PI structural gene is equivalent to the defined locus nmp and that the genes for PI class A and PI class B are alleles of the same locus. The PI class B gene of strain MS11 was cloned and sequenced, and comparison of this sequence with the gene sequence of PI class A of FA19 revealed a number of regions of significant divergence. By selection for the closely linked marker in transformations between the two strains, a series of strains with a hybrid PI was obtained. Analysis of these strains with monoclonal antibodies and oligonucleotides specific to PI class A or PI class B elucidated the nature and location of some of the surface-exposed epitopes, a thorough characterization of which is a prerequisite for understanding the role of PI in gonococcal pathogenesis and its possible use as a component of a vaccine.

MeSH Terms
Alleles Base Sequence Chromosome Mapping Epitopes/analysis Molecular Sequence Data Mutation Neisseria gonorrhoeae/genetics Nerve Tissue Proteins/genetics Synapsins
Chemicals
Epitopes Nerve Tissue Proteins Synapsins
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Carbonetti N H
Department of Microbiology and Immunology, University of North Carolina School of Medicine, Chapel Hill 27599.
Simnad V I
Seifert H S
So M
Sparling P F
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.85.18.6841
Published
1988-09-00
Pages
6841-5
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC282074
Subset
IM
Grants
NIAID NIH HHS · AI15036 · United States
NIAID NIH HHS · R37 AI26837 · United States
Databases
GENBANK
M21289
Corrections
ErratumIn
-
External Links
PubMed source DOI: 10.1073/pnas.85.18.6841 PMC Full Text View journal details
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