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PMID: 2456569 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Sequence analysis of cDNA and genomic DNA for a putative pertussis toxin-insensitive guanine nucleotide-binding regulatory protein alpha subunit.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 85 ·No. 15 ·1988-08-00 ·Pages 5384-8

Matsuoka M, Itoh H, Kozasa T, Kaziro Y

Abstract

We have isolated cDNA clones from rat C6 glioma cells coding for several guanine nucleotide-binding regulatory protein (G protein) alpha subunits (G alpha). The cDNA clones were then used to isolate human chromosomal genes. Among human genomic clones isolated by cross-hybridization with the rat cDNA for the alpha subunit of the inhibitory G protein Gi2, termed Gi2 alpha, a clone designated lambda HGi62 was found to contain a sequence that is highly homologous but distinct from any of the known G alpha sequences, and we have tentatively designated this sequence Gx alpha. We have searched a rat brain cDNA library with the Gx alpha sequence and isolated a cDNA clone containing a rat sequence similar to human Gx alpha. The cDNA contained a single open reading frame of 1065 nucleotides coding for a protein of 355 amino acids with a calculated molecular weight of 40,879. The amino acid sequence of rat Gx alpha shows 66% and 40% similarity with rat Gi2 alpha and rat Gs alpha (the alpha subunit of the stimulatory G protein), respectively. By RNA blot hybridization analysis, mRNA of approximately 3.2 kilobases was detected mainly in brain. Interestingly, the deduced amino acid sequence of Gx alpha predicts that the Gx alpha protein may be refractory to modification by pertussis toxin since the cysteine residue in the fourth position from the C terminus of pertussis toxin-sensitive G alpha is replaced by isoleucine.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Cloning, Molecular Cross Reactions DNA/genetics GTP-Binding Proteins/genetics Gene Expression Regulation Glioma Humans Molecular Sequence Data Nucleic Acid Hybridization Pertussis Toxin RNA/genetics Rats Tumor Cells, Cultured Virulence Factors, Bordetella/pharmacology
Chemicals
Virulence Factors, Bordetella RNA DNA Pertussis Toxin GTP-Binding Proteins
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Matsuoka M
Institute of Medical Science, University of Tokyo, Japan.
Itoh H
Kozasa T
Kaziro Y
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.85.15.5384
Published
1988-08-00
Pages
5384-8
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC281761
Subset
IM
Databases
GENBANK
J03773
External Links
PubMed source DOI: 10.1073/pnas.85.15.5384 PMC Full Text View journal details
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