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PMID: 2455823 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Vaccination against lethal coronavirus-induced encephalitis with a synthetic decapeptide homologous to a domain in the predicted peplomer stalk.

Journal of virology ·Vol. 62 ·No. 8 ·1988-08-00 ·Pages 3032-6

Talbot PJ, Dionne G, Lacroix M

Abstract

A surface probability method was used to select a decapeptide homologous to residues 993 to 1002 of the peplomer protein E2 of murine hepatitis virus strain JHM, a neurotropic coronavirus. This sequence of amino acids corresponded to a minor peak on a hydrophilicity plot. Immunization of mice with the chemically synthesized peptide coupled to keyhole limpet hemocyanin elicited high levels of neutralizing antibody and protected against lethal virus challenge. Protection correlated with a critical level of antipeptide antibody, which could be reached after a single inoculation. These results suggest that an appropriate antibody response to a highly restricted, surface-exposed domain of this viral protein is critical in determining the outcome of infection of the central nervous system. This sequence is located in the C-terminal fifth of the E2 peplomers, between two predicted coiled-coil structures.

MeSH Terms
Antibodies, Viral/biosynthesis Antigens, Viral/immunology Encephalitis, Arbovirus/prevention & control Epitopes Murine hepatitis virus/immunology Oligopeptides/chemical synthesis,immunology Protein Conformation Surface Properties Viral Envelope Proteins/immunology Viral Vaccines/immunology Water
Chemicals
Antibodies, Viral Antigens, Viral Epitopes Oligopeptides Viral Envelope Proteins Viral Vaccines Water
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Talbot P J
Virology Research Center, Université du Québec, Laval, Canada.
Dionne G
Lacroix M
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.62.8.3032-3036.1988
Published
1988-08-00
Pages
3032-6
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC253743
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.62.8.3032-3036.1988 PMC Full Text View journal details
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