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PMID: 24515776 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

MiR-148a regulates MEG3 in gastric cancer by targeting DNA methyltransferase 1.

Medical oncology (Northwood, London, England) ·Vol. 31 ·No. 3 ·2014-03-00 ·Pages 879

Yan J, Guo X, Xia J, Shan T, Gu C, Liang Z, Zhao W, Jin S

Abstract

The long non-coding RNA MEG3 has been reported to be a tumor suppressor in a number of malignant tumors including gastric cancer. Several studies have shown that the regulation of MEG3 may attribute to the promoter hypermethylation. However, the mechanism of MEG3 regulation in gastric cancer is still not well understood. MiR-148a can suppress gastric tumorigenesis through regulating the expression of target genes such as DNA methyltransferase 1(DNMT-1). We examined the expression of MEG3 in 52 gastric cancer samples using quantitative real-time PCR and found the down-regulation of MEG3 in both gastric cancer tissues and cell lines. The positive correlation of MEG3 and miR-148a was further confirmed in SGC-7901 and BGC-823 gastric cancer cell lines. Hypermethylation of MEG3 differentially methylated regions was identified by methylation-specific PCR, and MEG3 expression was increased with the inhibition of methylation with siRNA to DNMT-1 in gastric cancer cells. In addition, transfection of MEG3 siRNA into gastric cancer cells diminished the suppression of proliferation induced by overexpression of miR-148a. Our results suggest that the suppression of miR-148a may contribute to the down-regulation of MEG3 in gastric cancer by modulation of DNMT-1.

MeSH Terms
Blotting, Western Cell Proliferation DNA (Cytosine-5-)-Methyltransferase 1 DNA (Cytosine-5-)-Methyltransferases/genetics,metabolism DNA Methylation Gastric Mucosa/metabolism Gene Expression Regulation, Neoplastic Humans MicroRNAs/genetics Prognosis Promoter Regions, Genetic/genetics RNA, Long Noncoding/genetics RNA, Messenger/genetics Real-Time Polymerase Chain Reaction Reverse Transcriptase Polymerase Chain Reaction Stomach Neoplasms/genetics,metabolism,pathology
Chemicals
MEG3 non-coding RNA, human MIRN148 microRNA, human MicroRNAs RNA, Long Noncoding RNA, Messenger DNA (Cytosine-5-)-Methyltransferase 1 DNA (Cytosine-5-)-Methyltransferases
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Yan Jiang
Department of General Surgery, Nanjing Medical University Affiliated Wuxi Second Hospital, Wuxi, 214002, China.
Guo Xiaoqiang
Xia Jiazeng
Shan Tin
Gu Chen
Liang Zheng
Zhao Wei
Jin Shimao
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Article Info
Journal
Medical oncology (Northwood, London, England)
Abbr.
Med Oncol
ISSN
1559-131X
DOI
10.1007/s12032-014-0879-6
Published
2014-03-00
Epub
2014-00-11
Pages
879
Language
English
Region
United States
NLM ID
9435512
Subset
IM
External Links
PubMed source DOI: 10.1007/s12032-014-0879-6 View journal details
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