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PMID: 2449920 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Patch electrode glass composition affects ion channel currents.

Biophysical journal ·Vol. 53 ·No. 2 ·1988-02-00 ·Pages 287-92

Furman RE, Tanaka JC

Abstract

The influence of patch electrode glass composition on macroscopic IV relations in inside-out patches of the cGMP-activated ion channel from rod photoreceptors was examined for a soda lime glass, a Kovar sealing glass, a borosilicate glass, and several soft lead glasses. In several glasses the shape or magnitude of the currents changed as the concentration of EGTA or EDTA was increased from 200 microM to 10 mM. The changes in IV response suggest that, at low concentrations of chelator, divalent cations are released from the electrode glass and interact with the cGMP-activated channel. Possible mechanisms are discussed to explain the observations, and several comments are made concerning the choice of a glass for patching.

MeSH Terms
Animals Cyclic GMP/pharmacology Electrodes Glass In Vitro Techniques Ion Channels/drug effects,physiology Photoreceptor Cells/physiology Rana pipiens
Chemicals
Ion Channels Cyclic GMP
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Furman R E
Department of Neurology, University of Pennsylvania School of Medicine, Philadelphia 19104.
Tanaka J C
References (9)
9 references, click to expand
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Article Info
Journal
Biophysical journal
Abbr.
Biophys J
ISSN
0006-3495
DOI
10.1016/S0006-3495(88)83091-1
Published
1988-02-00
Pages
287-92
Language
English
Region
United States
NLM ID
0370626
PMCID
PMC1330150
Subset
IM
Grants
NEI NIH HHS · EY06640 · United States
NINDS NIH HHS · NS00865 · United States
NCRR NIH HHS · S07-RR-05415 · United States
External Links
PubMed source DOI: 10.1016/S0006-3495(88)83091-1 PMC Full Text View journal details
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