Home LiteratureArticle Details
PMID: 2443724 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

A 20S particle ubiquitous from yeast to human.

Journal of molecular evolution ·Vol. 25 ·No. 2 ·1987-00-00 ·Pages 141-50

Arrigo AP, Simon M, Darlix JL, Spahr PF

Abstract

We have purified and characterized a particle sedimenting at 20S from the postribosomal fraction of yeast, wheat germ, Drosophila melanogaster tissue culture cells, chicken embryo fibroblasts, rabbit reticulocyte lysate, and HeLa cells. Most of the protein constituents of the 20S particle have molecular weights of 20-35 kd and differ between species; however, some do have similar molecular weights and isoelectric points, suggesting they are related. Several low-molecular-weight RNAs, distinct from tRNAs, co-purify with the particle isolated from all these species and show increasingly more complex patterns ascending the arbitrary order from yeast to human (yeast, plant, insect, bird, and mammals). In Drosophila, we present evidence that these small RNAs are tightly associated with this 20S structure.

MeSH Terms
Animals Biological Evolution Chick Embryo Drosophila melanogaster/genetics Fibroblasts/ultrastructure Haplorhini/genetics HeLa Cells/ultrastructure Humans Plants/genetics RNA/genetics Rabbits/genetics Reticulocytes/ultrastructure Saccharomyces cerevisiae/genetics Species Specificity
Chemicals
RNA
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Arrigo A P
Cold Spring Harbor Laboratory, New York 11724.
Simon M
Darlix J L
Spahr P F
References (28)
28 references, click to expand
  1. Prosomes. Ubiquity and inter-species structural variation.
    J Mol Biol. 1986 Feb 20;187(4):479-93 PMID: 2423694
  2. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
    Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
  3. Purification and partial characterization of ring-shaped miniparticles.
    Cell Biol Int Rep. 1983 May;7(5):333-43 PMID: 6850864
  4. Optimization of conditions for labeling the 3' OH end of tRNA using T4 RNA ligase.
    Biochimie. 1983 Jun;65(6):367-70 PMID: 6412769
  5. Cytoplasmic particles and aminoacyl transferase I activity.
    J Cell Biol. 1970 Apr;45(1):1-8 PMID: 4917983
  6. Simian virus 40 and polyoma virus induce synthesis of heat shock proteins in permissive cells.
    Mol Cell Biol. 1983 Jan;3(1):1-8 PMID: 6298601
  7. Analysis of cytoplasmic 19 S ring-type particles in Drosophila which contain hsp 23 at normal growth temperature.
    Dev Biol. 1985 Jul;110(1):65-74 PMID: 2408945
  8. Immunofluorescence localization of a small heat shock protein (hsp 23) in salivary gland cells of Drosophila melanogaster.
    Mol Gen Genet. 1981;184(1):73-9 PMID: 6801431
  9. Eukaryotic pre-tRNA 5' processing nuclease: copurification with a complex cylindrical particle.
    Cell. 1986 Aug 1;46(3):377-85 PMID: 3637121
  10. Mapping adenines, guanines, and pyrimidines in RNA.
    Nucleic Acids Res. 1977 Aug;4(8):2527-38 PMID: 409999
  11. Electrophoretic characterization of bacterial polyribosomes in agarose-acrylamide composite gels.
    J Mol Biol. 1969 Apr 14;41(1):139-47 PMID: 4979520
  12. Preparative two-dimensional polyacrylamide gel electrophoresis of 32 P-labeled RNA.
    Anal Biochem. 1972 Sep;49(1):184-97 PMID: 5081097
  13. Specific labeling of 3' termini of RNA with T4 RNA ligase.
    Methods Enzymol. 1980;65(1):65-74 PMID: 6154874
  14. New rapid gel sequencing method for RNA.
    Nature. 1977 Oct 27;269(5631):833-6 PMID: 927512
  15. Characterization of the prosome from Drosophila and its similarity to the cytoplasmic structures formed by the low molecular weight heat-shock proteins.
    EMBO J. 1985 Feb;4(2):399-406 PMID: 2410257
  16. The 22 S cylinder particles of Xenopus laevis. I. Biochemical and electron microscopic characterization.
    Eur J Cell Biol. 1983 Nov;32(1):143-56 PMID: 6667691
  17. Subribosomal particles of Hela cells.
    Exp Cell Res. 1974 Aug;87(2):253-8 PMID: 4415975
  18. Investigation of the function of the heat shock proteins in Drosophila melanogaster tissue culture cells.
    Mol Gen Genet. 1980;178(3):517-24 PMID: 6156380
  19. Characterization of the genomic RNA from a Rous sarcoma virus mutant temperature sensitive for cell transformation.
    Nucleic Acids Res. 1979 Feb;6(2):471-85 PMID: 218172
  20. A cellular protein phosphorylated by the avian sarcoma virus transforming gene product is associated with ribonucleoprotein particles.
    EMBO J. 1983;2(3):309-15 PMID: 11894943
  21. Some negative contrast staining features of a protein from erythrocyte ghosts.
    J Mol Biol. 1969 Dec 14;46(2):329-35 PMID: 4188185
  22. Localization of the heat shock-induced proteins in Drosophila melanogaster tissue culture cells.
    Dev Biol. 1980 Jul;78(1):86-103 PMID: 6772504
  23. High resolution two-dimensional electrophoresis of proteins.
    J Biol Chem. 1975 May 25;250(10):4007-21 PMID: 236308
  24. Eukaryotic DNA segments capable of autonomous replication in yeast.
    Proc Natl Acad Sci U S A. 1980 Aug;77(8):4559-63 PMID: 6449009
  25. The 22 S cylinder particles of Xenopus laevis. II. Immunological characterization and localization of their proteins in tissues and cultured cells.
    Eur J Cell Biol. 1983 Nov;32(1):157-63 PMID: 6667692
  26. A new method for sequencing DNA.
    Proc Natl Acad Sci U S A. 1977 Feb;74(2):560-4 PMID: 265521
  27. Minute ring-shaped particles in cultured cells of malignant origin.
    Nat New Biol. 1973 May 30;243(126):146-50 PMID: 4515741
  28. The prosome: an ubiquitous morphologically distinct RNP particle associated with repressed mRNPs and containing specific ScRNA and a characteristic set of proteins.
    EMBO J. 1984 Jan;3(1):29-34 PMID: 6200323
Article Info
Journal
Journal of molecular evolution
Abbr.
J Mol Evol
ISSN
0022-2844
DOI
10.1007/BF02101756
Published
1987-00-00
Pages
141-50
Language
English
Region
Germany
NLM ID
0360051
Subset
IM
External Links
PubMed source DOI: 10.1007/BF02101756 View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2