Home LiteratureArticle Details
PMID: 2410257 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Characterization of the prosome from Drosophila and its similarity to the cytoplasmic structures formed by the low molecular weight heat-shock proteins.

The EMBO journal ·Vol. 4 ·No. 2 ·1985-02-00 ·Pages 399-406

Arrigo AP, Darlix JL, Khandjian EW, Simon M, Spahr PF

Abstract

We have identified and characterized a ribonucleoprotein structure from the cytoplasm of Drosophila melanogaster tissue culture cells which is equivalent to the prosome, a recently described ribonucleoprotein particle of duck and mouse cells. During the recovery period following heat shock, the low mol. wt. heat-shock proteins form cytoplasmic ribonucleoprotein particles which co-purify with the Drosophila prosome. Both ribonucleoprotein particles share several structural properties but their protein constituents differ in their metabolism and cellular localization during the heat treatment. We also report the partial nucleotide sequences of several small RNA species associated with the Drosophila prosome. One of them has a strong sequence homology with the U6 mammalian small nuclear RNA.

MeSH Terms
Animals Base Sequence Cell Compartmentation Cytoplasm/ultrastructure Drosophila melanogaster/physiology,ultrastructure Heat-Shock Proteins/immunology,metabolism Hot Temperature Isoelectric Point Macromolecular Substances Microscopy, Electron Molecular Weight RNA/analysis RNA, Small Nuclear Ribonucleoproteins/analysis,metabolism
Chemicals
Heat-Shock Proteins Macromolecular Substances RNA, Small Nuclear Ribonucleoproteins RNA
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Arrigo A P
Darlix J L
Khandjian E W
Simon M
Spahr P F
References (40)
40 references, click to expand
  1. The effect of heat shock on gene expression in Drosophila melanogaster.
    Cold Spring Harb Symp Quant Biol. 1978;42 Pt 2:819-27 PMID: 98271
  2. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
    Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
  3. Fluorographic detection of radioactivity in polyacrylamide gels with the water-soluble fluor, sodium salicylate.
    Anal Biochem. 1979 Sep 15;98(1):132-5 PMID: 543547
  4. Intracellular localization of heat shock proteins in Drosophila.
    Cell. 1980 Jul;20(3):679-89 PMID: 6774811
  5. Sequence of U1 RNA from Drosophila melanogaster: implications for U1 secondary structure and possible involvement in splicing.
    Nucleic Acids Res. 1981 Dec 11;9(23):6351-68 PMID: 6172778
  6. A comparison of the multiple Drosophila heat shock proteins in cell lines and larval salivary glands by two-dimensional gel electrophoresis.
    J Mol Biol. 1982 Jun 25;158(2):181-201 PMID: 6811757
  7. Nucleotide sequences of mouse genomic loci including a gene or pseudogene for U6 (4.8S) nuclear RNA.
    Nucleic Acids Res. 1981 Oct 10;9(19):5145-58 PMID: 6171774
  8. Simian virus 40 and polyoma virus induce synthesis of heat shock proteins in permissive cells.
    Mol Cell Biol. 1983 Jan;3(1):1-8 PMID: 6298601
  9. Immunofluorescence localization of a small heat shock protein (hsp 23) in salivary gland cells of Drosophila melanogaster.
    Mol Gen Genet. 1981;184(1):73-9 PMID: 6801431
  10. Mapping adenines, guanines, and pyrimidines in RNA.
    Nucleic Acids Res. 1977 Aug;4(8):2527-38 PMID: 409999
  11. Heat-shock proteins are associated with hnRNA in Drosophila melanogaster tissue culture cells.
    EMBO J. 1983;2(5):705-10 PMID: 16453451
  12. Heat-shock proteins of Drosophila are associated with nuclease-resistant, high-salt-resistant nuclear structures.
    J Cell Biol. 1981 Sep;90(3):793-6 PMID: 6793602
  13. Simian virus 40 large tumor antigen: a "RNA binding protein"?
    Proc Natl Acad Sci U S A. 1982 Feb;79(4):1139-43 PMID: 6175963
  14. Putative function of Drosophila melanogaster heat shock proteins in the nucleoskeleton.
    J Biol Chem. 1981 Nov 10;256(21):10735-8 PMID: 6793585
  15. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.
    Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350-4 PMID: 388439
  16. Electrophoretic characterization of bacterial polyribosomes in agarose-acrylamide composite gels.
    J Mol Biol. 1969 Apr 14;41(1):139-47 PMID: 4979520
  17. The heat shock response is self-regulated at both the transcriptional and posttranscriptional levels.
    Cell. 1982 Dec;31(3 Pt 2):593-603 PMID: 7159929
  18. Translational control of protein synthesis in response to heat shock in D. melanogaster cells.
    Cell. 1980 Dec;22(3):825-34 PMID: 6780199
  19. Different intracellular distributions of heat-shock and arsenite-induced proteins in Drosophila Kc cells. Possible relation with the phosphorylation and translocation of a major cytoskeletal protein.
    J Mol Biol. 1982 Dec 5;162(2):365-78 PMID: 6819361
  20. New rapid gel sequencing method for RNA.
    Nature. 1977 Oct 27;269(5631):833-6 PMID: 927512
  21. Differential repression of specific mRNA in erythroblast cytoplasm: a possible role for free mRNP proteins.
    EMBO J. 1983;2(11):1869-76 PMID: 6641704
  22. Investigation of the function of the heat shock proteins in Drosophila melanogaster tissue culture cells.
    Mol Gen Genet. 1980;178(3):517-24 PMID: 6156380
  23. Quantitative film detection of 3H and 14C in polyacrylamide gels by fluorography.
    Eur J Biochem. 1975 Aug 15;56(2):335-41 PMID: 1175627
  24. Characterization of the genomic RNA from a Rous sarcoma virus mutant temperature sensitive for cell transformation.
    Nucleic Acids Res. 1979 Feb;6(2):471-85 PMID: 218172
  25. A cellular protein phosphorylated by the avian sarcoma virus transforming gene product is associated with ribonucleoprotein particles.
    EMBO J. 1983;2(3):309-15 PMID: 11894943
  26. In vitro culture of Drosophila melanogaster embryonic cells.
    In Vitro. 1970 Nov-Dec;6(3):162-72 PMID: 5002052
  27. Localization of the heat shock-induced proteins in Drosophila melanogaster tissue culture cells.
    Dev Biol. 1980 Jul;78(1):86-103 PMID: 6772504
  28. The detection of DNA-binding proteins by protein blotting.
    Nucleic Acids Res. 1980 Jan 11;8(1):1-20 PMID: 6243775
  29. The nucleotide sequence of nuclear 4.8S RNA of mouse cells.
    Biochem Biophys Res Commun. 1980 Aug 14;95(3):1332-40 PMID: 6251836
  30. High resolution two-dimensional electrophoresis of proteins.
    J Biol Chem. 1975 May 25;250(10):4007-21 PMID: 236308
  31. High spontaneous mutation rate of Rous sarcoma virus demonstrated by direct sequencing of the RNA genome.
    Nucleic Acids Res. 1983 Sep 10;11(17):5953-67 PMID: 6310522
  32. Cross-linking of mRNA to proteins by irradiation of intact cells with ultraviolet light.
    Eur J Biochem. 1980 Nov;112(2):323-30 PMID: 7460925
  33. Nucleocytoplasmic distribution of snRNPs and stockpiled snRNA-binding proteins during oogenesis and early development in Xenopus laevis.
    Cell. 1983 Feb;32(2):425-34 PMID: 6186395
  34. The second Sir Hans Krebs Lecture. Informosomes.
    Eur J Biochem. 1969 Aug;10(1):20-35 PMID: 4899460
  35. Nucleotide sequence analysis of the Drosophila small heat shock gene cluster at locus 67B.
    J Mol Biol. 1983 Mar 25;165(1):35-57 PMID: 6302284
  36. A new method for sequencing DNA.
    Proc Natl Acad Sci U S A. 1977 Feb;74(2):560-4 PMID: 265521
  37. Four small Drosophila heat shock proteins are related to each other and to mammalian alpha-crystallin.
    Proc Natl Acad Sci U S A. 1982 Apr;79(7):2360-4 PMID: 6285380
  38. The nucleotide sequence of nuclear U6 (4.7 S) RNA.
    J Biol Chem. 1980 Sep 25;255(18):8901-6 PMID: 6773955
  39. The prosome: an ubiquitous morphologically distinct RNP particle associated with repressed mRNPs and containing specific ScRNA and a characteristic set of proteins.
    EMBO J. 1984 Jan;3(1):29-34 PMID: 6200323
  40. Electron microscopy study of 70 S ribosomes of Escherichia coli.
    FEBS Lett. 1971 May 10;14(4):203-205 PMID: 11945758
Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1985-02-00
Pages
399-406
Language
English
Region
England
NLM ID
8208664
PMCID
PMC554199
Subset
IM
External Links
PubMed source PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2