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PMID: 2442723 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Effects of UV, 4-NQO and TPA on gene expression in cultured human epidermal keratinocytes.

Nucleic acids research ·Vol. 15 ·No. 15 ·1987-08-11 ·Pages 5945-62

Kartasova T, Cornelissen BJ, Belt P, van de Putte P

Abstract

In an approach to study effects of UV light on gene expression in human epidermal keratinocytes, a cDNA library was constructed from poly(A)RNA isolated after UV irradiation from cultured keratinocytes. The cDNA library was differentially screened with labelled cDNA probes synthesized on poly(A)RNA isolated from UV irradiated or nonirradiated keratinocytes. Forty clones were selected and subjected to further analysis, 31 of them are described in this report. Whereas total mRNA synthesis is reduced after UV irradiation or treatment with 4-NQO Northern blot analysis revealed that there is an at least relative increase in the level of mRNAs corresponding to the majority of the isolated cDNA clones. Among these 15 were identified as corresponding to mRNAs for 50K and 56K keratins and for 50K- and 46K-related keratin. In addition, clones were found corresponding to the proteinase inhibitor cystatin A and to the glyceraldehyde 3-phosphate dehydrogenase (GAPDH). Treatment of keratinocytes with the tumor promoter TPA had no effect on the mRNA level for most of the clones except those corresponding to keratins. Our results indicate that in keratinocytes UV irradiation leads to a relative increase in the level of some mRNAs.

MeSH Terms
4-Nitroquinoline-1-oxide/pharmacology Amino Acid Sequence Base Sequence Cells, Cultured Cysteine Proteinase Inhibitors DNA/isolation & purification Epidermis/drug effects,metabolism,radiation effects Humans Keratins/genetics Nitroquinolines/pharmacology Nucleic Acid Hybridization Protease Inhibitors/genetics RNA, Messenger/biosynthesis,drug effects,radiation effects Tetradecanoylphorbol Acetate/pharmacology
Chemicals
Cysteine Proteinase Inhibitors Nitroquinolines Protease Inhibitors RNA, Messenger 4-Nitroquinoline-1-oxide Keratins DNA Tetradecanoylphorbol Acetate
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Kartasova T
Cornelissen B J
Belt P
van de Putte P
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49 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/15.15.5945
Published
1987-08-11
Pages
5945-62
Language
English
Region
England
NLM ID
0411011
PMCID
PMC306060
Subset
IM
Databases
GENBANK
X05803, X05978
External Links
PubMed source DOI: 10.1093/nar/15.15.5945 PMC Full Text View journal details
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