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PMID: 2442286 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Mouse H-2k-restricted cytotoxic T cells recognize antigenic determinants in both the HA1 and HA2 subunits of the influenza A/PR/8/34 hemagglutinin.

The Journal of experimental medicine ·Vol. 166 ·No. 3 ·1987-09-01 ·Pages 693-701

Gould KG, Scotney H, Townsend AR, Bastin J, Brownlee GG

Abstract

We have constructed two chimeric influenza hemagglutinin (HA) genes in which the HA1 and HA2 subunits of the HA molecule have been interchanged between influenza A/PR/8/34 (H1 subtype) and A/NT/60/68 (H3 subtype). These genes were used to construct recombinant vaccinia viruses that expressed intact chimeric HA. These recombinant viruses were used to test whether murine CTL recognize antigenic determinants in either the HA1, HA2, or both subunits. We found that both subunits of the HA molecule contain determinants for CTL. This implies that CTL have, at least in part, separate antigenic determinants from B lymphocytes, which recognize mainly epitopes within the HA1 subunit.

MeSH Terms
Animals Antigens, Viral/immunology DNA/genetics DNA, Recombinant Epitopes/immunology Female H-2 Antigens/immunology Hemagglutinins/genetics,immunology Influenza A virus/genetics,immunology Mice Mice, Inbred CBA T-Lymphocytes, Cytotoxic/immunology Vaccinia virus/genetics
Chemicals
Antigens, Viral DNA, Recombinant Epitopes H-2 Antigens H-2K(K) antigen Hemagglutinins DNA
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Gould K G
Scotney H
Townsend A R
Bastin J
Brownlee G G
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32 references, click to expand
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Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.166.3.693
Published
1987-09-01
Pages
693-701
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2188694
Subset
IM
External Links
PubMed source DOI: 10.1084/jem.166.3.693 PMC Full Text View journal details
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