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PMID: 2433044 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Diazepam action on gamma-aminobutyric acid-activated chloride currents in internally perfused frog sensory neurons.

Cellular and molecular neurobiology ·Vol. 6 ·No. 3 ·1986-09-00 ·Pages 307-23

Hattori K, Oomura Y, Akaike N

Abstract

The Cl- current (ICl) in gamma-aminobutyric acid (GABA)-sensitive frog sensory neuron was separated from other Na+, Ca2+, and K+ currents using a suction pipette technique which allows internal perfusion under a single-electrode voltage clamp. Diazepam (DZP) itself evoked no response but facilitated the dose- and time-dependently GABA-induced ICl without changing the GABA equilibrium potential (EGABA) at concentrations ranging widely, from 3 X 10(-9) to 10(-4) M. In the presence of DZP, the GABA dose-response curve shifted to the left without changing the maximum current, indicating that DZP modifies the interaction between GABA and its receptor rather than affecting directly the channel activation step. The enhancement of the GABA-induced ICl by DZP depended neither on the membrane voltage nor on the inward or outward direction of the ICl. DZP also potentiated the ICl elicited by GABA agonists such as beta-alanine, taurine, homotaurine, 5-aminovaleric acid, l-GABOB, d-GABOB, glycine, and muscimol. The GABA response enhanced by pentobarbital (PB) was further enhanced by adding DZP, indicating that DZP and PB do not act in the same way. Ro5-3663, a diazepam analogue, enhanced the GABA-induced ICl only in a narrow range of the concentrations but inhibited the current at concentrations higher than 2 X 10(-6) M.

MeSH Terms
Animals Benzodiazepinones/administration & dosage Chlorides/metabolism Diazepam/administration & dosage Drug Interactions Ganglia, Spinal/drug effects In Vitro Techniques Ion Channels/drug effects Membrane Potentials/drug effects Neurons, Afferent/drug effects Pentobarbital/administration & dosage Rana catesbeiana gamma-Aminobutyric Acid/administration & dosage
Chemicals
Benzodiazepinones Chlorides Ion Channels gamma-Aminobutyric Acid Ro 5-3663 Pentobarbital Diazepam
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Hattori K
Oomura Y
Akaike N
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Article Info
Journal
Cellular and molecular neurobiology
Abbr.
Cell Mol Neurobiol
ISSN
0272-4340
DOI
10.1007/BF00711116
Published
1986-09-00
Pages
307-23
Language
English
Region
United States
NLM ID
8200709
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00711116 View journal details
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