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PMID: 6323715 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Separation of ionic currents in the somatic membrane of frog sensory neurons.

The Journal of membrane biology ·Vol. 78 ·No. 1 ·1984-00-00 ·Pages 19-28

Ishizuka S, Hattori K, Akaike N

Abstract

Electrical properties of isolated frog primary afferent neurons were examined by suction pipette technique, which combines internal perfusion with current or voltage clamp using a switching circuit with a single electrode. When K+ in the external and internal solutions was totally replaced with Cs+, extremely prolonged Ca spikes, lasting for 5 to 10 sec, and Na spikes, having a short plateau phase of 10 to 15 msec, were observed in Na+-free and Ca2+-free solutions, respectively. Under voltage clamp, Ca2+ current (ICa) appeared at around -30 mV and maximum peak current was elicited at about 0 mV. With increasing test pulses to the positive side, ICa became smaller and flattened but did not reverse. Increases of [Ca]o induced a hyperbolic increase of ICa and also shifted its I-V curve along the voltage axis to the more positive direction. Internal perfusion of F- blocked ICa time-dependently. The Ca channel was permeable to foreign divalent cations in the sequence of ICa greater than IBa greater than ISr much greater than IMn greater than IZn. Organic Ca-blockers equally depressed the divalent cation currents dose- and time-dependently without shifting the I-V relationships, while inorganic blockers suppressed these currents dose-dependently and the inhibition appeared much stronger in the order of IBa = ISr greater than ICa greater than IMn = IZn.

MeSH Terms
Action Potentials/drug effects Animals Calcium/pharmacology Calcium Channel Blockers/pharmacology Cations/pharmacology Conductometry/instrumentation Ganglia, Spinal/cytology Ion Channels/metabolism Membrane Potentials/drug effects Neurons, Afferent/drug effects,physiology Potassium/pharmacology Rana catesbeiana/physiology
Chemicals
Calcium Channel Blockers Cations Ion Channels Potassium Calcium
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Ishizuka S
Hattori K
Akaike N
References (45)
45 references, click to expand
  1. Zinc-dependent action potentials in giant neurons of the snail, Euhadra quaestia.
    J Membr Biol. 1979 Sep 14;49(4):325-44 PMID: 480340
  2. Further analysis of inhibitory effects of propranolol and local anaesthetics on the calcium current in Helix neurones.
    Br J Pharmacol. 1982 May;76(1):37-43 PMID: 7082906
  3. Long-lasting inward current in snail neurons in barium solutions in voltage-clamp conditions.
    J Membr Biol. 1977 Jul 14;35(3):239-56 PMID: 886608
  4. Three pharmacologically distinct potassium channels in molluscan neurones.
    J Physiol. 1977 Feb;265(2):465-88 PMID: 850203
  5. Voltage clamp of the Aplysia giant neurone: early sodium and calcium currents.
    J Physiol. 1970 Nov;211(1):217-44 PMID: 5500995
  6. Calcium current-dependent and voltage-dependent inactivation of calcium channels in Helix aspersa.
    J Physiol. 1981 Nov;320:193-218 PMID: 6275075
  7. Sr action potentials in cardiac Purkynĕ fibres. I. Evidence for a regenerative increase in Sr conductance.
    Pflugers Arch. 1971;322(1):60-72 PMID: 5099621
  8. Membrane responses of frog's spinal ganglion cells in calcium-free solutions.
    J Physiol. 1962 Aug;163:1-12 PMID: 14457913
  9. The calcium current of Helix neuron.
    J Gen Physiol. 1978 May;71(5):509-31 PMID: 660160
  10. Properties of internally perfused, voltage-clamped, isolated nerve cell bodies.
    J Gen Physiol. 1978 May;71(5):489-507 PMID: 660159
  11. A transient outward current in a mammalian central neurone blocked by 4-aminopyridine.
    Nature. 1982 Sep 16;299(5880):252-4 PMID: 6287290
  12. Effect of internal fluoride and phosphate on membrane currents during intracellular dialysis of nerve cells.
    Nature. 1975 Oct 23;257(5528):691-3 PMID: 1186845
  13. Voltage clamping with a single microelectrode.
    J Neurobiol. 1975 Jul;6(4):411-22 PMID: 1181381
  14. Calcium current in molluscan neurones: measurement under conditions which maximize its visibility.
    J Physiol. 1979 Jan;286:41-60 PMID: 439033
  15. Primary afferent neurones: the ionic mechanism of GABA-mediated depolarization.
    Neuropharmacology. 1974 Mar;13(3):215-9 PMID: 4546218
  16. Membrane currents carried by Ca, Sr, and Ba in barnacle muscle fiber during voltage clamp.
    J Gen Physiol. 1974 May;63(5):564-78 PMID: 4824996
  17. The electrical activity of spinal ganglion cells investigated with intracellular microelectrodes.
    Jpn J Physiol. 1957 Dec 20;7(4):297-323 PMID: 13501950
  18. Evidence of non-specificity of the Ca channel in mammalian myocardial fibre membranes. Substitution of Ca by Sr, Ba or Mg as charge carriers.
    Pflugers Arch. 1973 Aug 17;342(2):125-36 PMID: 4795633
  19. The selectivity of ion channels in nerve and muscle.
    Neuroscience. 1982 Jun;7(6):1335-66 PMID: 6289166
  20. Inhibitory effects of propranolol on the calcium current of Helix neurones.
    Br J Pharmacol. 1981 Jun;73(2):431-4 PMID: 6263388
  21. Measurement of calcium influx under voltage clamp in molluscan neurones using the metallochromic dye arsenazo III.
    J Physiol. 1979 Jan;286:61-82 PMID: 439040
  22. Calcium channel.
    Annu Rev Neurosci. 1981;4:69-125 PMID: 6261668
  23. Inotropic and electrophysiological actions of verapamil and D 600 in mammalian myocardium. II. Pattern of inotropic effects of the optical isomers.
    Naunyn Schmiedebergs Arch Pharmacol. 1975;290(1):69-80 PMID: 1178070
  24. Aequorin response facilitation and intracellular calcium accumulation in molluscan neurones.
    J Physiol. 1980 Mar;300:167-96 PMID: 6247486
  25. Surface density of calcium ions and calcium spikes in the barnacle muscle fiber membrane.
    J Gen Physiol. 1967 Jan;50(3):583-601 PMID: 11526848
  26. Characteristics of sodium and calcium conductance changes produced by membrane depolarization in an Aplysia neurone.
    J Physiol. 1979 Apr;289:143-61 PMID: 458645
  27. Divalent cations as charge carriers in excitable membranes.
    Prog Biophys Mol Biol. 1973;26:1-43 PMID: 4575321
  28. Divalent ion currents and the delayed potassium conductance in an Aplysia neurone.
    J Physiol. 1980 Jul;304:297-313 PMID: 6255142
  29. Calcium currents in internally perfused nerve cell bodies of Limnea stagnalis.
    J Physiol. 1982 Jan;322:503-28 PMID: 7069629
  30. Voltage clamp studies of a transient outward membrane current in gastropod neural somata.
    J Physiol. 1971 Feb;213(1):21-30 PMID: 5575340
  31. A quantitative description of membrane current and its application to conduction and excitation in nerve.
    J Physiol. 1952 Aug;117(4):500-44 PMID: 12991237
  32. Separation of sodium and calcium currents in the somatic membrane of mollusc neurones.
    J Physiol. 1977 Sep;270(3):545-68 PMID: 903906
  33. Changes in the intracellular concentration of free calcium ions in a pace-maker neurone, measured with the metallochromic indicator dye arsenazo III.
    J Physiol. 1978 Feb;275:357-76 PMID: 633127
  34. Effect of quaternary ammonium ions on electrical activity of spinal ganglion cells in frogs.
    J Neurophysiol. 1959 Mar;22(2):177-94 PMID: 13642094
  35. The ionic requirements for the production of action potentials in crustacean muscle fibres.
    J Physiol. 1958 Aug 6;142(3):516-43 PMID: 13576452
  36. Mapping of nerve cells in the suboesophageal ganglia of Helix aspersa.
    Comp Biochem Physiol A Comp Physiol. 1975 Jan 1;50(1A):1-25 PMID: 234036
  37. Zinc current in Helix soma membrane.
    Comp Biochem Physiol C. 1982;72(2):403-10 PMID: 6128163
  38. Voltage-dependent activation of potassium current in Helix neurones by endogenous cellular calcium.
    J Physiol. 1983 Jan;334:309-24 PMID: 6408248
  39. Actions of verapamil, diltiazem and other divalent cations on the calcium-current of Helix neurones.
    Br J Pharmacol. 1981 Sep;74(1):87-95 PMID: 7272605
  40. A voltage-sensitive persistent calcium conductance in neuronal somata of Helix.
    J Physiol. 1976 Jan;254(1):129-51 PMID: 1249721
  41. Sodium current in single heart muscle cells.
    Nature. 1979 Mar 15;278(5701):269-71 PMID: 423978
  42. The units of calcium conduction in Helix neurones.
    Nature. 1978 Jul 27;274(5669):379-82 PMID: 672962
  43. Ionic currents through the membrane of the mammalian oocyte and their comparison with those in the tunicate and sea urchin.
    J Physiol. 1977 May;267(2):465-95 PMID: 559759
  44. Differentiation of the transmembrane Na and Ca channels in mammalian cardiac fibres by the use of specific inhibitors.
    Pflugers Arch. 1972;335(4):309-22 PMID: 4673213
  45. Membrane currents of the tunicate egg under the voltage-clamp condition.
    J Physiol. 1976 Jan;254(3):607-38 PMID: 943522
Article Info
Journal
The Journal of membrane biology
Abbr.
J Membr Biol
ISSN
0022-2631
DOI
10.1007/BF01872528
Published
1984-00-00
Pages
19-28
Language
English
Region
United States
NLM ID
0211301
Subset
IM
External Links
PubMed source DOI: 10.1007/BF01872528 View journal details
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