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PMID: 2426706 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Single nisoldipine-sensitive calcium channels in smooth muscle cells isolated from rabbit mesenteric artery.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 83 ·No. 15 ·1986-08-00 ·Pages 5746-50

Worley JF, Deitmer JW, Nelson MT

Abstract

Single smooth muscle cells were enzymatically isolated from the rabbit mesenteric artery. At physiological levels of external Ca, these cells were relaxed and contracted on exposure to norepinephrine, caffeine, or high levels of potassium. The patch-clamp technique was used to measure unitary currents through single channels in the isolated cells. Single channels were selective for divalent cations and exhibited two conductance levels, 8 pS and 15 pS. Both types of channels were voltage-dependent, and channel activity occurred at potentials positive to -40 mV. The activity of both channel types was almost completely inhibited by 50 nM nisoldipine. These channels appear to be the pathways for voltage-dependent Ca influx in vascular smooth muscle and may be the targets of the clinically used dihydropyridines.

MeSH Terms
Animals Calcium/physiology Cell Separation Electric Conductivity In Vitro Techniques Ion Channels/drug effects,physiology Membrane Potentials Mesenteric Arteries Muscle, Smooth, Vascular/drug effects,physiology Nifedipine/analogs & derivatives,pharmacology Nisoldipine Rabbits Sarcolemma/physiology
Chemicals
Ion Channels Nisoldipine Nifedipine Calcium
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Worley J F
Deitmer J W
Nelson M T
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28 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.83.15.5746
Published
1986-08-00
Pages
5746-50
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC386366
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.83.15.5746 PMC Full Text View journal details
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