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PMID: 2426572 Published · ppublish English Journal Article

Developmentally regulated cytokeratin gene in Xenopus laevis.

Molecular and cellular biology ·Vol. 5 ·No. 10 ·1985-10-00 ·Pages 2575-81

Winkles JA, Sargent TD, Parry DA, Jonas E, Dawid IB

Abstract

We have determined the sequence of cloned cDNAs derived from a 1,665-nucleotide mRNA which transiently accumulates during Xenopus laevis embryogenesis. Computer analysis of the deduced amino acid sequence revealed that this mRNA encodes a 47-kilodalton type I intermediate filament subunit, i.e., a cytokeratin. As is common to all intermediate filament subunits so far examined, the predicted polypeptide, named XK70, contains N- and C-terminal domains flanking a central alpha-helical rod domain. The overall amino acid homology between XK70 and a human 50-kilodalton type I keratin is 47%; homology within the alpha-helical domain is 57%. The N-terminal domain, which is not completely contained in our cDNAs, is basic, contains 42% serine plus alanine, and includes five copies of a six-amino-acid repeating unit. The C-terminal domain has a high alpha-helical content and contains a region with sequence homology to the C-terminal domains of other type I and type III intermediate filament proteins. We suggest that different keratin filament subtypes may have different functional roles during amphibian oogenesis and embryogenesis.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Cloning, Molecular DNA/genetics Gene Expression Regulation Keratins/genetics Molecular Weight RNA, Messenger/genetics Xenopus laevis/embryology,genetics
Chemicals
RNA, Messenger Keratins DNA
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Winkles J A
Sargent T D
Parry D A
Jonas E
Dawid I B
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45 references, click to expand
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.5.10.2575-2581.1985
Published
1985-10-00
Pages
2575-81
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC366992
Subset
IM
Databases
GENBANK
M11032
External Links
PubMed source DOI: 10.1128/mcb.5.10.2575-2581.1985 PMC Full Text View journal details
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