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PMID: 24253170 Published · ppublish English Journal Article

Molecular cloning and characterisation of cDNAs complementary to mRNAs from wounded potato (Solanum tuberosum) tuber tissue.

Planta ·Vol. 162 ·No. 4 ·1984-10-00 ·Pages 353-60

Shirras AD, Northcote DH

Abstract

Five cDNA clones complementary to mRNAs representing different abundances and responses to wounding have been isolated from a library of Sau 3A fragments in the bacteriophage M13 mp8. These were characterised by hybrid-release translation and hybridisation to RNA blots. The levels of RNA complementary to two of the clones show a marked increase during the 24h after wounding, one shows a small increase and two show no appreciable changes except that caused by a general increase in the total amount of polyadenylated RNA per microgram of total RNA which increases 2.5-fold during the same period. The would-induced RNAs are not induced in diluted suspension-culture cells, but RNA complementary to each clone is present in varying levels in stems, leaves and roots of intact potato plants.

Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Shirras A D
Department of Biochemistry, University of Cambridge, Tennis Court Road, CB2 1QW, Cambridge, UK.
Northcote D H
References (31)
31 references, click to expand
  1. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
    Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
  2. Fat metabolism in higher plants. XXXIV. Development of fatty acid synthetase as a function of protein synthesis in aging potato tuber slices.
    Plant Physiol. 1967 Mar;42(3):391-7 PMID: 6045298
  3. Screening lambdagt recombinant clones by hybridization to single plaques in situ.
    Science. 1977 Apr 8;196(4286):180-2 PMID: 322279
  4. Coordinated changes in enzyme activities of phenylpropanoid metabolism during the growth of soybean cell suspension cultures.
    Biochim Biophys Acta. 1974 Oct 8;362(3):417-24 PMID: 4472044
  5. Time course and spatial distribution of phenylalanine ammonia-lyase and peroxidase activity in wounded potato tuber tissue.
    Plant Physiol. 1978 Nov;62(5):789-93 PMID: 16660607
  6. Phenylalanine ammonia-lyase and cinnamic acid 4-hydroxylase: Characterisation of the concomitant changes in enzyme activities in illuminated potato tuber discs.
    Planta. 1977 Jan;135(2):169-75 PMID: 24420020
  7. Polyribosome formation and RNA synthesis during aging of carrot-root tissue.
    Proc Natl Acad Sci U S A. 1967 May;57(5):1338-44 PMID: 5231738
  8. Method for detection of specific RNAs in agarose gels by transfer to diazobenzyloxymethyl-paper and hybridization with DNA probes.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5350-4 PMID: 414220
  9. Enhanced autoradiographic detection of 32P and 125I using intensifying screens and hypersensitized film.
    FEBS Lett. 1977 Oct 15;82(2):314-6 PMID: 913604
  10. Aromatic metabolism in plants. II. Enzymes of the shikimate pathway in suspension cultures of plant cells.
    Can J Biochem. 1966 Jun;44(6):791-9 PMID: 5919280
  11. Activation of ribosomal and messenger RNA synthesis in excised Jerusalem artichoke tuber slices.
    Planta. 1977 Jan;136(3):203-10 PMID: 24420392
  12. Dictyostelium 17S, 25S, and 5S rDNAs lie within a 38,000 base pair repeated unit.
    Cell. 1976 Nov;9(3):431-8 PMID: 1033038
  13. Induction of Phenylalanine Deaminase by Light and its Relation to Chlorogenic Acid Synthesis in Potato Tuber Tissue.
    Plant Physiol. 1965 Sep;40(5):779-84 PMID: 16656157
  14. The making of strand-specific M13 probes.
    Gene. 1982 Mar;17(3):271-7 PMID: 7049837
  15. Sequential Induction of Phenylalanine Ammonia-lyase and a Lyase-inactivating System in Potato Tuber Disks.
    Plant Physiol. 1968 Mar;43(3):365-74 PMID: 16656772
  16. Characterisation of bacterial clones containing DNA sequences derived from Xenopus laevis vitellogenin mRNA.
    Nucleic Acids Res. 1979 Feb;6(2):487-506 PMID: 424303
  17. Characterization of Carrot Cell Wall Protein : I. EFFECT OF alpha, alpha'-DIPYRIDYL ON CELL WALL PROTEIN SYNTHESIS AND SECRETION IN INCUBATED CARROT DISCS.
    Plant Physiol. 1981 Oct;68(4):956-63 PMID: 16662033
  18. Sensitive detection of RNA using strand-specific M13 probes.
    Gene. 1982 Dec;20(2):139-44 PMID: 6187624
  19. An efficient mRNA-dependent translation system from reticulocyte lysates.
    Eur J Biochem. 1976 Aug 1;67(1):247-56 PMID: 823012
  20. Specific effects on enzyme activities upon ditution of petroselinum hortense cell cultures into water.
    Arch Biochem Biophys. 1975 Dec;171(2):500-6 PMID: 1200637
  21. Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose.
    Proc Natl Acad Sci U S A. 1980 Sep;77(9):5201-5 PMID: 6159641
  22. Synthesis of rRNA, tRNA and other RNA-species concomitant with polyribosome formation in aging potato tuber slices.
    Z Naturforsch B. 1971 Oct;26(10):1058-64 PMID: 4401632
  23. Quantitative film detection of 3H and 14C in polyacrylamide gels by fluorography.
    Eur J Biochem. 1975 Aug 15;56(2):335-41 PMID: 1175627
  24. Alteration of Coding Properties of Polysome-Associated Messenger RNA in Potato Tuber Slices during Aging.
    Plant Physiol. 1981 Jul;68(1):154-7 PMID: 16661861
  25. The location of arabinosyl:hydroxyproline transferase in the membrane system of potato tissue culture cells.
    Biochem J. 1981 Jun 1;195(3):661-7 PMID: 6797402
  26. Subculture-induced protein synthesis in tissue cultures of Glycine max and Phaseolus vulgaris.
    Planta. 1981 May;152(1):24-31 PMID: 24302314
  27. Cloning in single-stranded bacteriophage as an aid to rapid DNA sequencing.
    J Mol Biol. 1980 Oct 25;143(2):161-78 PMID: 6260957
  28. Structure, gas chromatographic measurement, and function of suberin synthesized by potato tuber tissue slices.
    Plant Physiol. 1974 Jul;54(1):116-21 PMID: 16658825
  29. Utilization of stored messenger RNA during early aging of Jerusalem artichoke tuber slices.
    Planta. 1978 Jan;143(1):75-83 PMID: 24408264
  30. The effects of infection by Phytophthora infestans on the control of phenylpropanoid metabolism in wounded potato tissue.
    Planta. 1981 May;151(6):535-40 PMID: 24302205
  31. Qualitative and quantitative changes in mRNA of castor beans during the initial stages of germination.
    Planta. 1981 Feb;151(2):189-97 PMID: 24301728
Article Info
Journal
Planta
Abbr.
Planta
ISSN
0032-0935
DOI
10.1007/BF00396748
Published
1984-10-00
Pages
353-60
Language
English
Region
Germany
NLM ID
1250576
External Links
PubMed source DOI: 10.1007/BF00396748 View journal details
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