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PMID: 24213266 Published · ppublish English Journal Article

Chromosomal localization and genomic organization of α-amylase genes in rice (Oryza sativa L.).

TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik ·Vol. 82 ·No. 4 ·1991-07-00 ·Pages 481-8

Ranjhan S, Litts JC, Foolad MR, Rodriguez RL

Abstract

Genes for α-amylase, alcohol dehydrogenase, andEm, an ABA-regulated gene expressed late in embryogenesis, were localized on rice chromosomes by the analysis of primary trisomies. The validity of the mapping approach was confirmed usingAdh-1 as a control. TheAdh-1 gene has previously been assigned to chromosome 11 using conventional techniques. In this study we confirm this assignment and report an additional locus for alcohol dehydrogenase (Adh-2) on chromosome 9. The α-amylase genes were located on chromosomes 1, 2, 6, 8, and 9 while theEm gene was mapped to chromosome 5. To facilitate trisomic analysis and correlation of cloned genes with bands observed on Southern blots, a nomenclature for the rice α-amylase genes has been proposed. In addition to mapping nine cloned α-amylase genes, we have identified two previously uncloned α-amylase genes as part of this study. Polymorphism for α-amylase genes belonging to each of the three subfamilies was observed between M202 and IR36. The maximum degree of polymorphism was found among genes belonging to the RAmy3 subfamily, which also has the most diverse group of genes.

Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Ranjhan S
Department of Genetics, University of California, 95616, Davis, CA, USA.
Litts J C
Foolad M R
Rodriguez R L
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Article Info
Journal
TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik
Abbr.
Theor Appl Genet
ISSN
0040-5752
DOI
10.1007/BF00588603
Published
1991-07-00
Pages
481-8
Language
English
Region
Germany
NLM ID
0145600
External Links
PubMed source DOI: 10.1007/BF00588603 View journal details
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