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PMID: 24213166 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Quantifying the local resolution of cryo-EM density maps.

Nature methods ·Vol. 11 ·No. 1 ·2014-01-00 ·Pages 63-5

Kucukelbir A, Sigworth FJ, Tagare HD

Abstract

We propose a definition of local resolution for three-dimensional electron cryo-microscopy (cryo-EM) density maps that uses local sinusoidal features. Our algorithm has no free parameters and is applicable to other imaging modalities, including tomography. By evaluating the local resolution of single-particle reconstructions and subtomogram averages for four example data sets, we report variable resolution across a 4- to 40-Å range.

MeSH Terms
Algorithms Alleles Cryoelectron Microscopy/methods Fourier Analysis Imaging, Three-Dimensional Likelihood Functions Models, Theoretical Normal Distribution Proteins/chemistry Ribosomes/chemistry Software Viruses/chemistry
Chemicals
Proteins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Kucukelbir Alp
Department of Biomedical Engineering, Yale University, New Haven, Connecticut, USA.
Sigworth Fred J
1] Department of Biomedical Engineering, Yale University, New Haven, Connecticut, USA. [2] Department of Cellular and Molecular Physiology, Yale University, New Haven, Connecticut, USA.
Tagare Hemant D
1] Department of Biomedical Engineering, Yale University, New Haven, Connecticut, USA. [2] Department of Diagnostic Radiology, Yale University, New Haven, Connecticut, USA.
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12 references, click to expand
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Article Info
Journal
Nature methods
Abbr.
Nat Methods
ISSN
1548-7105
DOI
10.1038/nmeth.2727
Published
2014-01-00
Epub
2013-00-10
Pages
63-5
Language
English
Region
United States
NLM ID
101215604
PMCID
PMC3903095
Subset
IM
Grants
NLM NIH HHS · R01LM010142 · United States
NLM NIH HHS · R01 LM010142 · United States
NINDS NIH HHS · R01 NS021501 · United States
NIGMS NIH HHS · R01 GM095658 · United States
NINDS NIH HHS · R01NS021501 · United States
NIGMS NIH HHS · R01GM095658 · United States
External Links
PubMed source DOI: 10.1038/nmeth.2727 PMC Full Text View journal details
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