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PMID: 20637413 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Review

Definition and estimation of resolution in single-particle reconstructions.

Structure (London, England : 1993) ·Vol. 18 ·No. 7 ·2010-07-14 ·Pages 768-75

Liao HY, Frank J

Abstract

In this paper, we review current practices for establishing the resolution in single-particle reconstructions. The classical Raleigh criterion for the resolution is not applicable in this case, and the resolution is commonly defined by a consistency test, whereby the data set is randomly split in half and the two resulting reconstructions are then compared. Such a procedure, however, may introduce statistical dependence between the two half-sets, which leads to a too optimistic resolution estimate. On the other hand, this overestimation is counteracted by the diminished statistical properties of a mere half of the data set. The "true" resolution of the whole data set can be estimated when the functional relationship between the data size and the resolution is known. We are able to estimate this functional by taking into account the B-factor and the geometry of data collection. Finally, the drawbacks of resolution estimation are entirely avoided by computing the correlation of neighboring voxels in the Fourier domain.

MeSH Terms
Crystallography/methods Fourier Analysis Image Processing, Computer-Assisted/methods Microscopy/methods Models, Theoretical Optical Phenomena Particle Size
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Liao Hstau Y
Department of Biochemistry and Molecular Biophysics, Columbia University, New York, NY 10032, USA.
Frank Joachim
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Article Info
Journal
Structure (London, England : 1993)
Abbr.
Structure
ISSN
1878-4186
DOI
10.1016/j.str.2010.05.008
Published
2010-07-14
Pages
768-75
Language
English
Region
United States
NLM ID
101087697
PMCID
PMC2923553
Subset
IM
Grants
NIGMS NIH HHS · R01 GM029169 · United States
NIGMS NIH HHS · R01 GM029169-29 · United States
Howard Hughes Medical Institute · United States
NIGMS NIH HHS · R01 GM029169-28 · United States
NIGMS NIH HHS · R37 GM029169 · United States
NIGMS NIH HHS · R37 GM29169 · United States
External Links
PubMed source DOI: 10.1016/j.str.2010.05.008 PMC Full Text View journal details
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