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PMID: 2419896 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Colony probing as an alternative to standard sequencing as a means of direct analysis of chromosomal DNA to determine the spectrum of single-base changes in regions of known sequence.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 83 ·No. 4 ·1986-02-00 ·Pages 1026-30

Miller JK, Barnes WM

Abstract

We demonstrate here the means to directly analyze bacterial chromosomal DNA for all classes of single-base mutations in a specific codon in any region of known sequence through the use of DNA probing as a powerful substitute for standard sequencing techniques. With this method, chromosomal DNA from hundreds of mutants can be examined for single-base changes without any DNA cloning. This method can conveniently provide a large data base for the assessment of all classes of single-base mutations occurring spontaneously or induced by a known or suspected mutagen. The method is demonstrated for the analysis of histidine-independent (His+) revertants of hisG46, a missense mutation of Salmonella typhimurium that can revert in six or seven ways.

MeSH Terms
Azacitidine/pharmacology Bacteriological Techniques Base Sequence Chromosomes, Bacterial/analysis Codon DNA, Bacterial/analysis Genes, Bacterial Genetic Markers Genetic Techniques Mutation Nucleic Acid Hybridization Oligodeoxyribonucleotides/chemical synthesis,genetics Salmonella typhimurium/drug effects,genetics
Chemicals
Codon DNA, Bacterial Genetic Markers Oligodeoxyribonucleotides Azacitidine
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Miller J K
Barnes W M
References (18)
18 references, click to expand
  1. Colony hybridization: a method for the isolation of cloned DNAs that contain a specific gene.
    Proc Natl Acad Sci U S A. 1975 Oct;72(10):3961-5 PMID: 1105573
  2. The complete pattern of mutagenesis arising from the repair of site-specific psoralen crosslinks: analysis by oligonucleotide hybridization.
    Nucleic Acids Res. 1984 Dec 21;12(24):9237-48 PMID: 6514576
  3. A simple method for detecting environmental carcinogens as mutagens.
    Ann N Y Acad Sci. 1976;271:5-13 PMID: 793475
  4. Genetic studies of the lac repressor. I. Correlation of mutational sites with specific amino acid residues: construction of a colinear gene-protein map.
    J Mol Biol. 1977 Jan 15;109(2):275-98 PMID: 839542
  5. Genetic studies of the lac repressor. IV. Mutagenic specificity in the lacI gene of Escherichia coli.
    J Mol Biol. 1977 Dec 15;117(3):577-606 PMID: 416218
  6. Evidence for transposition of dispersed repetitive DNA families in yeast.
    Cell. 1979 Apr;16(4):739-51 PMID: 378399
  7. Hybridization of synthetic oligodeoxyribonucleotides to phi chi 174 DNA: the effect of single base pair mismatch.
    Nucleic Acids Res. 1979 Aug 10;6(11):3543-57 PMID: 158748
  8. Filter replicas and permanent collections of recombinant DNA plasmids.
    Nucleic Acids Res. 1979 Dec 20;7(8):2115-36 PMID: 118435
  9. The use of synthetic oligonucleotides as hybridization probes. II. Hybridization of oligonucleotides of mixed sequence to rabbit beta-globin DNA.
    Nucleic Acids Res. 1981 Feb 25;9(4):879-94 PMID: 7232206
  10. Genetic analysis of the histidine operon control region of Salmonella typhimurium.
    J Mol Biol. 1981 Feb 5;145(4):713-34 PMID: 7021855
  11. Shotgun DNA sequencing using cloned DNase I-generated fragments.
    Nucleic Acids Res. 1981 Jul 10;9(13):3015-27 PMID: 6269069
  12. Oligonucleotide-directed mutagenesis as a general and powerful method for studies of protein function.
    Proc Natl Acad Sci U S A. 1982 Nov;79(21):6409-13 PMID: 6983070
  13. DNA sequence changes of mutations in the histidine operon control region that decrease attenuation.
    J Mol Biol. 1983 Apr 15;165(3):443-59 PMID: 6302291
  14. Mutagenicity of 5-azacytidine in Salmonella typhimurium.
    Mutat Res. 1983 Jul;121(1):1-6 PMID: 6191215
  15. An improved colony hybridization method with significantly increased sensitivity for detection of single genes.
    Plasmid. 1983 Nov;10(3):296-8 PMID: 6657780
  16. Mutational specificity of depurination.
    Proc Natl Acad Sci U S A. 1984 Mar;81(5):1494-8 PMID: 6369329
  17. Spontaneous mutation frequencies in Salmonella: enhancement of G/C to A/T transitions and depression of deletion and frameshift mutation frequencies afforded by anoxic incubation.
    Environ Mutagen. 1984;6(5):633-50 PMID: 6383792
  18. Methods for detecting carcinogens and mutagens with the Salmonella/mammalian-microsome mutagenicity test.
    Mutat Res. 1975 Dec;31(6):347-64 PMID: 768755
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.83.4.1026
Published
1986-02-00
Pages
1026-30
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC323003
Subset
IM
Grants
NIGMS NIH HHS · GM 07067 · United States
NIGMS NIH HHS · GM24956 · United States
External Links
PubMed source DOI: 10.1073/pnas.83.4.1026 PMC Full Text View journal details
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