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PMID: 2419588 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Herpes simplex virus specifies two subunits of ribonucleotide reductase encoded by 3'-coterminal transcripts.

Journal of virology ·Vol. 57 ·No. 3 ·1986-03-00 ·Pages 802-8

Swain MA, Galloway DA

Abstract

We have previously described a transcription unit located between map coordinates 0.558 and 0.595 on the herpes simplex virus type 2 strain 333 genome which encodes two mRNAs of 5.0 and 1.2 kilobases that share a common 3' terminus, and we have determined the nucleotide sequence of a 38,000-dalton protein specified by the smaller RNA (D. A. Galloway and M. A. Swain, J. Virol. 49:724-730, 1984). The entire nucleotide sequence of the 140,000-dalton protein specified by a 3,432-base-pair open reading frame within the large mRNA is presented, as are transcriptional regulatory sequences upstream of the RNA. The 140,000-dalton protein shows strong homology with the large subunit of well-characterized ribonucleotide reductase enzymes from the mouse and from Escherichia coli and with an Epstein-Barr virus gene. The 38,000-dalton protein has been shown previously to have homology with the small subunit of these enzymes (B.-M. Sjoberg, H. Eklund, J. A. Fuchs, J. Carlson, N. M. Standart, J. V. Ruderman, S. J. Bray, and T. Hunt, FEBS Lett. 183:99-102, 1985). This is the first example of a herpesvirus transcriptional unit that encodes functionally related proteins.

MeSH Terms
Amino Acid Sequence Antibodies, Monoclonal/immunology Base Sequence Cell Transformation, Viral DNA, Viral/analysis Epitopes/analysis Molecular Weight Ribonucleotide Reductases/analysis,genetics,immunology Simplexvirus/enzymology,genetics Transcription, Genetic Viral Proteins/analysis
Chemicals
Antibodies, Monoclonal DNA, Viral Epitopes Viral Proteins Ribonucleotide Reductases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Swain M A
Galloway D A
References (39)
39 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.57.3.802-808.1986
Published
1986-03-00
Pages
802-8
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC252808
Subset
IM
Grants
NCI NIH HHS · CA 35568 · United States
Databases
GENBANK
K02023, K02911, M12700
External Links
PubMed source DOI: 10.1128/JVI.57.3.802-808.1986 PMC Full Text View journal details
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