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PMID: 2418438 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

RecA protein filaments can juxtapose DNA ends: an activity that may reflect a function in DNA repair.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 83 ·No. 3 ·1986-02-00 ·Pages 624-8

Register JC, Griffith J

Abstract

To further characterize the role of RecA protein-DNA filaments in general recombination and DNA repair, we have examined interactions of these filaments with themselves following formation. When linear double-stranded DNA was incubated with RecA in the presence of Mg2+ and adenosine 5'-[gamma-thio]triphosphate, monomer-length (1n) nucleoprotein filaments were observed. Following continued incubation, filaments having 2n, 3n, ... lengths were observed, indicating that an end-to-end joining of the monomer-length filaments had occurred. When linear single-stranded DNA was covered by RecA protein under several conditions, the ends of the resulting filaments joined together rapidly, producing circular filaments. The end-to-end joining of single-stranded DNA-RecA filaments appeared to require that 3' DNA ends be juxtaposed with 5' DNA ends, because double-stranded DNA molecules having long single-stranded DNA tails with only 3' or 5' termini did not join end-to-end. However, when both 5' and 3' ends were present in the reaction, joining was observed. We suggest that this end-to-end joining activity may help explain the role of RecA protein in both the protection of damaged DNA ends and the repair of double-stranded DNA breaks.

MeSH Terms
DNA Repair DNA, Bacterial/metabolism DNA, Single-Stranded/metabolism Escherichia coli/physiology Microscopy, Electron RNA/metabolism Rec A Recombinases/metabolism
Chemicals
DNA, Bacterial DNA, Single-Stranded RNA Rec A Recombinases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Register J C
Griffith J
References (36)
36 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.83.3.624
Published
1986-02-00
Pages
624-8
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC322916
Subset
IM
Grants
NCI NIH HHS · CA16086 · United States
NIGMS NIH HHS · GM31819 · United States
External Links
PubMed source DOI: 10.1073/pnas.83.3.624 PMC Full Text View journal details
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