Isolation, characterization, phosphorylation and site of synthesis of Spinacia chloroplast ribosomal proteins.

Current genetics ·Vol. 8 ·No. 2 ·1984-02-00 ·Pages 147-54

Posno M, van Noort M, Débise R, Groot GS

Abstract

We have characterized the ribosomal proteins from Spinacia chloroplasts using two-dimensional gel electrophoresis. The 30S and 50S subunits contain 23-25 and 36 ribosomal proteins, respectively. In contrast to prokaryotic ribosomes, chloroplast ribosomes contain at least one (and possibly two) phosphorylated ribosomal proteins. Isolated chloroplasts synthesize in the presence of ((35)S) labeled methionine and cysteine at least seven 30S and thirteen 50S ribosomal proteins which are assembled into (pre)ribosomes. This suggests that about one third of the chloroplast ribosomal proteins is encoded by the chloroplast DNA itself. The identity of several labeled proteins in the two-dimensional gel electrophoretic patterns which did not comigrate with stained chloroplast ribosomal proteins is discussed.

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Posno M

Biochemical Laboratory, Free University, de Boelelaan 1085, 1081 HV, Amsterdam, The Netherlands.

van Noort M

Débise R

Groot G S

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Article Info

Journal

Current genetics

Abbr.

Curr Genet

ISSN

0172-8083

DOI

10.1007/BF00420227

Published

1984-02-00

Pages

147-54

Language

English

Region

United States

NLM ID

8004904

External Links

PubMed source DOI: 10.1007/BF00420227 View journal details

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Isolation, characterization, phosphorylation and site of synthesis of Spinacia chloroplast ribosomal proteins.

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