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PMID: 24173279 Published · ppublish English Journal Article

Both the chloroplast and nuclear genomes of Chlamydomonas reinhardi share homology with Escherichia coli genes for transcriptional and translational components.

Current genetics ·Vol. 7 ·No. 3 ·1983-06-00 ·Pages 201-10

Watson JC, Surzycki SJ

Abstract

Considerable DNA sequence homology can be detected between the Escherichia coli genes coding for translational and transcriptional components and both the chloroplast and nuclear genomes of Chlamydomonas reinhardi. Labeled chloroplast DNA was demonstrated to hybridize to DNA fragments of the transducing phages λfus3 and λspc2 that encode ribosomal proteins of the α and S10 operons. Further, chloroplast DNA probes hybridize to fragments of λrtf (d) 18 that encode the β and β' subunits of RNA polymerase. The regions homologous to the ribosomal protein and RNA polymerase genes were located on the chloroplast DNA physical map by probing restriction fragments of chloroplast DNA with phage or plasmid fragments carrying these E. coli genes. Probing nuclear DNA with bacterial gene probes revealed DNA fragments homologous to elongation factor and ribosomal protein genes. Most surprisingly, sequences homologous to the β subunit of RNA polymerase were found not only in chloroplast DNA but in nuclear DNA as well.

Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Watson J C
Department of Biology, Indiana University, 47405, Bloomington, Indiana, USA.
Surzycki S J
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Article Info
Journal
Current genetics
Abbr.
Curr Genet
ISSN
0172-8083
DOI
10.1007/BF00434891
Published
1983-06-00
Pages
201-10
Language
English
Region
United States
NLM ID
8004904
External Links
PubMed source DOI: 10.1007/BF00434891 View journal details
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