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PMID: 24169763 Published · ppublish English Journal Article

Molecular marker analysis of Helianthus annuus L. 2. Construction of an RFLP linkage map for cultivated sunflower.

TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik ·Vol. 91 ·No. 2 ·1995-07-00 ·Pages 195-9

Berry ST, Leon AJ, Hanfrey CC, Challis P, Burkholz A, Barnes SR, Rufener GK, Lee M, Caligari PD

Abstract

A detailed linkage map of Helianthus annuus was constructed based on segregation at 234 RFLP loci, detected by 213 probes, in an F2 population of 289 individuals (derived from a cross between the inbred lines HA89 and ZENB8). The genetic markers covered 1380 centiMorgans (cM) of the sunflower genome and were aranged in 17 linkage groups, corresponding to the haploid number of chromosomes in this species. One locus was found to be unlinked. Although the average interval size was 5.9 cM, there were a number of regions larger than 20 cM that were devoid of markers. Genotypic classes at 23 loci deviated significantly from the expected ratios (1∶2∶1 or 3∶1), all showing a reduction in the ZENB8 homozygous class. The majority of these loci were found to map to four regions on linkage groups G, L and P.

Authors & Affiliations
9 authors, click to expand affiliations / ORCID
Berry S T
ZENECA Seeds, Plant Biotechnology, Jealott's Hill Research Station, RG12 6EY, Bracknell, Berkshire, UK.
Leon A J
Hanfrey C C
Challis P
Burkholz A
Barnes S R
Rufener G K
Lee M
Caligari P D
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Article Info
Journal
TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik
Abbr.
Theor Appl Genet
ISSN
0040-5752
DOI
10.1007/BF00220877
Published
1995-07-00
Pages
195-9
Language
English
Region
Germany
NLM ID
0145600
External Links
PubMed source DOI: 10.1007/BF00220877 View journal details
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