Home LiteratureArticle Details
PMID: 2409533 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Small nuclear RNAs in the ciliate Tetrahymena.

Nucleic acids research ·Vol. 13 ·No. 11 ·1985-06-11 ·Pages 4203-24

Pedersen N, Hellung-Larsen P, Engberg J

Abstract

We have isolated and partially characterized a family of small nuclear RNAs (snRNAs) from three different species of the protozoan Tetrahymena. We find six distinct snRNAs ranging in size from 100 to 250 nucleotides. The two largest snRNAs, as well as an abundant, heterogenous group of smaller snRNAs are found in the nucleolar RNA fraction. None of the snRNAs are transcription products of the ribosomal RNA gene or its flanking regions, as shown by hybridization tests. The snRNAs are metabolically stable as determined by pulse/chase experiments and several of them contain a number of modified nuclotides. The snRNAs from Tetrahymena all have slightly different sizes from mammalian snRNAs. The cap structure of the snRNAs from Tetrahymena differs from that of the snRNAs from mammalian cells, but has not yet been fully characterized. The relative amount of snRNAs to total RNA is less in Tetrahymena (greater than 0.1%) than in mammalian cells (2%).

MeSH Terms
Animals Molecular Weight Nucleic Acid Hybridization RNA/biosynthesis,isolation & purification RNA Caps/isolation & purification RNA, Small Nuclear Species Specificity Tetrahymena/genetics Tetrahymena pyriformis/genetics Transcription, Genetic
Chemicals
RNA Caps RNA, Small Nuclear RNA
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Pedersen N
Hellung-Larsen P
Engberg J
References (46)
46 references, click to expand
  1. Low molecular weight RNA associated with 28 s nucleolar RNA.
    J Mol Biol. 1970 Feb 14;47(3):505-15 PMID: 5418169
  2. Characterization of rapidly labeled RNA in Tetrahymena pyriformis.
    Exp Cell Res. 1971 Jul;67(1):124-8 PMID: 5569195
  3. Small molecular weight RNA components in Tetrahymena pyriformis.
    Biochim Biophys Acta. 1971 Nov 29;254(1):78-90 PMID: 5002560
  4. Small molecular weight RNA components in Ehrlich ascites tumor cells.
    Biochim Biophys Acta. 1972 Mar 24;262(3):290-307 PMID: 4537696
  5. Cell multiplication in Tetrahymena cultures after addition of particulate material.
    J Cell Sci. 1973 Jan;12(1):275-86 PMID: 4632140
  6. Electrophoretic characterization of shuttling and nonshuttling small nuclear RNAs.
    Cell. 1974 Aug;2(4):259-69 PMID: 4369984
  7. Translation in vitro of vesicular stomatitis virus mRNA lacking 5'-terminal 7-methylguanosine.
    Nature. 1976 Jul 1;262(5563):32-7 PMID: 180425
  8. Isolation of the ribosomal RNA gene from Tetrahymena in the state of transcriptionally active chromatin.
    Biochem Biophys Res Commun. 1976 SEP 20;72(2):720-31 PMID: 825121
  9. The differential inhibitory effect of alpha-amanitin on the synthesis of low molecular weight RNA components in BHK cells.
    FEBS Lett. 1978 Mar 15;87(2):227-31 PMID: 631339
  10. Metabolism of low molecular weight ribonucleic acids in early sea urchin embryos.
    Biochemistry. 1979 Apr 3;18(7):1353-60 PMID: 427117
  11. Isolation and characterization of an RNA-containing nuclear matrix from Tetrahymena macronuclei.
    Biochemistry. 1979 May 1;18(9):1782-8 PMID: 107967
  12. Use of in vitro 32P labeling in the sequence analysis of nonradioactive tRNAs.
    Methods Enzymol. 1979;59:58-109 PMID: 220499
  13. Localization of transcribed regions on extrachromosomal ribosomal RNA genes of Tetrahymena thermophila by R-loop mapping.
    Proc Natl Acad Sci U S A. 1979 Oct;76(10):5051-5 PMID: 291921
  14. Antibodies to small nuclear RNAs complexed with proteins are produced by patients with systemic lupus erythematosus.
    Proc Natl Acad Sci U S A. 1979 Nov;76(11):5495-9 PMID: 316537
  15. Detection of specific RNAs or specific fragments of DNA by fractionation in gels and transfer to diazobenzyloxymethyl paper.
    Methods Enzymol. 1979;68:220-42 PMID: 94421
  16. The nucleotide sequence of nuclear U6 (4.7 S) RNA.
    J Biol Chem. 1980 Sep 25;255(18):8901-6 PMID: 6773955
  17. Electrophoretic transfer of DNA, RNA and protein onto diazobenzyloxymethyl (DBM) - paper.
    Nucleic Acids Res. 1980 Jan 25;8(2):299-317 PMID: 6158735
  18. Dictyostelium small nuclear RNA D2 is homologous to rat nucleolar RNA U3 and is encoded by a dispersed multigene family.
    Cell. 1980 Nov;22(1 Pt 1):109-18 PMID: 6159098
  19. The chromatography of RNA and oligoribonucleotides on boronate-substituted agarose and polyacrylamide.
    Anal Biochem. 1980 Sep 1;107(1):128-35 PMID: 6159804
  20. The small nuclear RNAs of the cellular slime mold Dictyostelium discoideum. Isolation and characterization.
    J Biol Chem. 1981 Jan 25;256(2):956-63 PMID: 6161130
  21. Characterization and subcellular localization of 7-8 S RNAs of Novikoff hepatoma.
    J Biol Chem. 1981 Aug 25;256(16):8452-7 PMID: 6167578
  22. Isolation and characterization of small nuclear RNAs from Dictyostelium discoideum.
    J Biochem. 1981 Aug;90(2):299-308 PMID: 7298590
  23. Collection of published 5S and 5.8S RNA sequences and their precursors.
    Nucleic Acids Res. 1982 Jan 22;10(2):r93-115 PMID: 6174939
  24. Small nuclear RNAs are encoded in the nontranscribed region of ribosomal spacer DNA.
    Proc Natl Acad Sci U S A. 1982 May;79(10):3106-10 PMID: 6179077
  25. Signal recognition particle contains a 7S RNA essential for protein translocation across the endoplasmic reticulum.
    Nature. 1982 Oct 21;299(5885):691-8 PMID: 6181418
  26. m-Aminophenylboronate agarose specifically binds capped snRNA and mRNA.
    Nucleic Acids Res. 1982 Dec 11;10(23):7621-33 PMID: 6185925
  27. Complex endonucleolytic cleavage pattern during early events in the processing of pre-rRNA in the lower eukaryote, Tetrahymena thermophila.
    Nucleic Acids Res. 1983 Jun 11;11(11):3487-502 PMID: 6304633
  28. Fate of an intervening sequence ribonucleic acid: excision and cyclization of the Tetrahymena ribosomal ribonucleic acid intervening sequence in vivo.
    Biochemistry. 1983 May 10;22(10):2390-7 PMID: 6860634
  29. The U1 small nuclear RNA-protein complex selectively binds a 5' splice site in vitro.
    Cell. 1983 Jun;33(2):509-18 PMID: 6190573
  30. Nucleotide sequence of Dictyostelium small nuclear RNA Dd8 not homologous to any other sequenced small nuclear RNA.
    J Biol Chem. 1983 Sep 10;258(17):10606-13 PMID: 6193114
  31. A model for the involvement of the small nucleolar RNA (U3) in processing eukaryotic ribosomal RNA.
    Mol Biol Rep. 1983 May;9(1-2):75-8 PMID: 6193411
  32. Recognition signals for mouse pre-rRNA processing. A potential role for U3 nucleolar RNA.
    Mol Biol Rep. 1983 May;9(1-2):79-86 PMID: 6193412
  33. Electrophoretic elution of nucleic acids from acrylamide and agarose gels.
    J Biochem Biophys Methods. 1983 Jul;7(4):277-84 PMID: 6194199
  34. Splicing of messenger RNA precursors is inhibited by antisera to small nuclear ribonucleoprotein.
    Cell. 1983 Nov;35(1):101-7 PMID: 6194895
  35. Isolation and partial characterization of dinoflagellate U1-U6 small RNAs homologous to rat U small nuclear RNAs.
    J Biol Chem. 1983 Nov 25;258(22):13965-9 PMID: 6196358
  36. Yeast contains small nuclear RNAs encoded by single copy genes.
    Cell. 1983 Dec;35(3 Pt 2):743-51 PMID: 6197183
  37. A U4-like small nuclear RNA is dispensable in yeast.
    Cell. 1983 Dec;35(3 Pt 2):753-62 PMID: 6197184
  38. Nucleotide sequence of the 5'-terminal coding region for pre-rRNA and mature 17S rRNA in Tetrahymena thermophila rDNA.
    Nucleic Acids Res. 1984 Jan 25;12(2):959-72 PMID: 6320127
  39. Small nuclear RNAs and RNA processing.
    Prog Nucleic Acid Res Mol Biol. 1983;30:127-62 PMID: 6198692
  40. Primary and secondary structure of dinoflagellate U5 small nuclear RNA.
    Nucleic Acids Res. 1984 Feb 10;12(3):1529-42 PMID: 6199742
  41. High evolutionary conservation of the secondary structure and of certain nucleotide sequences of U5 RNA.
    Nucleic Acids Res. 1983 Dec 10;11(23):8359-67 PMID: 6200827
  42. U1, U2 and U5 small nuclear RNAs are found in plants cells. Complete nucleotide sequence of the U5 RNA family from pea nuclei.
    Nucleic Acids Res. 1983 Dec 20;11(24):8583-94 PMID: 6200829
  43. Ribonucleoprotein organization of eukaryotic RNA. XXX. Evidence that U1 small nuclear RNA is a ribonucleoprotein when base-paired with pre-messenger RNA in vivo.
    J Mol Biol. 1984 Apr 5;174(2):285-95 PMID: 6201617
  44. U4 and U6 RNAs coexist in a single small nuclear ribonucleoprotein particle.
    Nucleic Acids Res. 1984 Apr 11;12(7):3283-93 PMID: 6201826
  45. Occurrence and properties of low molecular weight RNA components from cells at different taxonomic levels.
    Comp Biochem Physiol B. 1977;58(3):273-81 PMID: 318268
  46. In vitro splicing of the ribosomal RNA precursor of Tetrahymena: involvement of a guanosine nucleotide in the excision of the intervening sequence.
    Cell. 1981 Dec;27(3 Pt 2):487-96 PMID: 6101203
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/13.11.4203
Published
1985-06-11
Pages
4203-24
Language
English
Region
England
NLM ID
0411011
PMCID
PMC341306
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/13.11.4203 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2