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PMID: 2406460 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Structural requirements for trans activation of human immunodeficiency virus type 1 long terminal repeat-directed gene expression by tat: importance of base pairing, loop sequence, and bulges in the tat-responsive sequence.

Journal of virology ·Vol. 64 ·No. 3 ·1990-03-00 ·Pages 1402-6

Roy S, Parkin NT, Rosen C, Itovitch J, Sonenberg N

Abstract

In order to elucidate the molecular mechanisms of action of the tat-responsive sequence, mutational analysis of the tat-responsive sequence was carried out. The most critical region comprised nucleotides +18 to +44 and included the 3-nucleotide bulge at positions +23 to +25, the loop sequence, and an intact stem. In addition, base pairing up to nucleotide +52 was required for the full magnitude of the trans-activation response. Single-nucleotide bulges at positions +5 to +17 were dispensable. Analysis of truncated and full-length transcripts demonstrated that a transcriptional antitermination model does not fully account for trans activation.

MeSH Terms
Base Composition Base Sequence Gene Expression Regulation, Viral Gene Products, tat/metabolism Genes, Viral HIV-1/genetics HeLa Cells/metabolism Humans Molecular Sequence Data Mutation Nucleic Acid Conformation RNA, Messenger/genetics Repetitive Sequences, Nucleic Acid Restriction Mapping Ribonucleases Trans-Activators/metabolism Transcriptional Activation Transfection tat Gene Products, Human Immunodeficiency Virus
Chemicals
Gene Products, tat RNA, Messenger Trans-Activators tat Gene Products, Human Immunodeficiency Virus Ribonucleases
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Roy S
Department of Biochemistry, McGill University, Montreal, Canada.
Parkin N T
Rosen C
Itovitch J
Sonenberg N
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.64.3.1402-1406.1990
Published
1990-03-00
Pages
1402-6
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC249266
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.64.3.1402-1406.1990 PMC Full Text View journal details
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