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PMID: 2404869 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

The 34-kilodalton membrane immunogen of Treponema pallidum is a lipoprotein.

Infection and immunity ·Vol. 58 ·No. 2 ·1990-02-00 ·Pages 384-92

Swancutt MA, Radolf JD, Norgard MV

Abstract

Treponema pallidum subsp. pallidum and Escherichia coli incorporated exogenous [3H]palmitate into the 34-kilodalton (kDa) pathogen-specific antigen of T. pallidum. Radiolabeled fatty acid remained associated with the protein upon immunoprecipitation and after boiling in sodium dodecyl sulfate, acetone precipitation, and extensive extractions in organic solvents, suggesting that the fatty acid was covalently bound to the protein. Detection of [3H]palmitate after alkaline and acid hydrolyses confirmed the identity of the incorporated label. Globomycin inhibited maturation of the recombinant 34-kDa antigen, suggesting that E. coli uses the lipoprotein-specific signal peptidase II to process the treponemal antigen. Globomycin also inhibited processing of the 34-kDa antigen, as well as the 44.5- and 15-kDa antigens, in T. pallidum, implying that T. pallidum also possesses the lipoprotein export pathway common to both gram-negative and gram-positive bacteria. Ethanol inhibited processing of the 34-kDa antigen in minicells, suggesting that the 34-kDa antigen normally is translocated through the cytoplasmic membrane. Comparison of the Triton X-114 phase partitioning behavior of the 34-kDa antigen produced either by minicells or by a cell-free translation system indicated that the covalent attachment of fatty acid conferred hydrophobic biochemical properties to the 34-kDa antigen, consistent with the hypothesis that the attached lipid anchors the 34-kDa antigen into the membrane.

MeSH Terms
Acylation Anti-Bacterial Agents Antigens, Bacterial/analysis,biosynthesis,immunology Escherichia coli/genetics,immunology Ethanol/pharmacology Fatty Acids/pharmacology Lipoproteins/analysis Octoxynol Peptides/pharmacology Polyethylene Glycols/pharmacology Precipitin Tests Treponema pallidum/immunology
Chemicals
Anti-Bacterial Agents Antigens, Bacterial Fatty Acids Lipoproteins Peptides Ethanol Polyethylene Glycols globomycin Octoxynol Nonidet P-40
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Swancutt M A
Department of Microbiology, University of Texas, Southwestern Medical Center, Dallas 75235.
Radolf J D
Norgard M V
References (44)
44 references, click to expand
  1. A single amino acid determinant of the membrane localization of lipoproteins in E. coli.
    Cell. 1988 May 6;53(3):423-32 PMID: 3284654
  2. Monoclonal antibody selection and analysis of a recombinant DNA-derived surface immunogen of Treponema pallidum expressed in Escherichia coli.
    Infect Immun. 1986 Apr;52(1):110-9 PMID: 3514451
  3. Selective release of the Treponema pallidum outer membrane and associated polypeptides with Triton X-114.
    J Bacteriol. 1988 Dec;170(12):5789-96 PMID: 3056914
  4. Sequence analysis of the 47-kilodalton major integral membrane immunogen of Treponema pallidum.
    Infect Immun. 1989 Jan;57(1):196-203 PMID: 2642466
  5. Outer membrane ultrastructure explains the limited antigenicity of virulent Treponema pallidum.
    Proc Natl Acad Sci U S A. 1989 Mar;86(6):2051-5 PMID: 2648388
  6. Major integral membrane protein immunogens of Treponema pallidum are proteolipids.
    Infect Immun. 1989 Sep;57(9):2872-7 PMID: 2668191
  7. Acylation of the 47-kilodalton major membrane immunogen of Treponema pallidum determines its hydrophobicity.
    Infect Immun. 1989 Sep;57(9):2878-85 PMID: 2668192
  8. Molecular characterization of the pathogen-specific, 34-kilodalton membrane immunogen of Treponema pallidum.
    Infect Immun. 1989 Nov;57(11):3314-23 PMID: 2680970
  9. Large exopenicillinase, initial extracellular form detected in cultures of Bacillus licheniformis.
    Biochemistry. 1980 Apr 29;19(9):1882-6 PMID: 6966510
  10. Purification and characterization of leader (signal) peptidase from Escherichia coli.
    J Biol Chem. 1980 Aug 25;255(16):7973-7 PMID: 6995457
  11. A synthetic analogue of Escherichia coli lipoprotein, tripalmitoyl pentapeptide, constitutes a potent immune adjuvant.
    J Immunol. 1986 Oct 15;137(8):2676-81 PMID: 3531339
  12. Cloning and expression of the major 47-kilodalton surface immunogen of Treponema pallidum in Escherichia coli.
    Infect Immun. 1986 Nov;54(2):500-6 PMID: 3021631
  13. Immunochemical characterization and purification of Treponema pallidum antigen TpD expressed by Escherichia coli K12.
    Sex Transm Dis. 1986 Oct-Dec;13(4):237-44 PMID: 3544257
  14. Nucleotide sequence of the gene for the peptidoglycan-associated lipoprotein of Escherichia coli K12.
    Eur J Biochem. 1987 Feb 16;163(1):73-7 PMID: 3545827
  15. Interaction of mitogenic bacterial lipoprotein and a synthetic analogue with mouse lymphocytes. Isolation and characterization of binding proteins.
    Eur J Biochem. 1987 Feb 2;162(3):651-7 PMID: 3549292
  16. Changes in the cell surface properties of Treponema pallidum that occur during in vitro incubation of freshly extracted organisms.
    Infect Immun. 1987 Sep;55(9):2255-61 PMID: 3305368
  17. Myristoylation and the post-translational acquisition of hydrophobicity by the membrane immunoglobulin heavy-chain polypeptide in B lymphocytes.
    Proc Natl Acad Sci U S A. 1987 Nov;84(21):7654-8 PMID: 3118373
  18. Cloning and expression in Escherichia coli of the gene encoding the heat-modifiable major outer membrane protein of Haemophilus influenzae type b.
    Infect Immun. 1987 Dec;55(12):2993-3000 PMID: 2824380
  19. Genes encoding two lipoproteins in the leuS-dacA region of the Escherichia coli chromosome.
    J Bacteriol. 1987 Dec;169(12):5692-9 PMID: 3316191
  20. Genetic and physicochemical characterization of the recombinant DNA-derived 47-kilodalton surface immunogen of Treponema pallidum subsp. pallidum.
    Infect Immun. 1988 Jan;56(1):71-8 PMID: 3275588
  21. Identification and localization of integral membrane proteins of virulent Treponema pallidum subsp. pallidum by phase partitioning with the nonionic detergent triton X-114.
    Infect Immun. 1988 Feb;56(2):490-8 PMID: 3276627
  22. Cloning of genes encoding a 15,000-dalton peptidoglycan-associated outer membrane lipoprotein and an antigenically related 15,000-dalton protein from Haemophilus influenzae.
    J Bacteriol. 1988 Feb;170(2):489-98 PMID: 2828309
  23. Covalent binding of lipid to protein. Diglyceride and amide-linked fatty acid at the N-terminal end of the murein-lipoprotein of the Escherichia coli outer membrane.
    Eur J Biochem. 1973 Apr;34(2):284-96 PMID: 4575979
  24. The lipoprotein of the outer membrane of Escherichia coli: a B-lymphocyte mitogen.
    J Exp Med. 1975 Aug 1;142(2):473-82 PMID: 1095681
  25. Biosynthesis and assembly of envelope lipoprotein in a glycerol-requiring mutant of Salmonella typhimurium.
    J Bacteriol. 1976 Mar;125(3):892-904 PMID: 767331
  26. Induction of lymphocyte proliferation and membrane changes by lipopeptide derivatives of the lipoprotein from the outer membrane of Escherichia coli.
    Z Immunitatsforsch Immunobiol. 1977 Apr;153(1):11-22 PMID: 325934
  27. Globomycin, a new peptide antibiotic with spheroplast-forming activity. II. Isolation and physico-chemical and biological characterization.
    J Antibiot (Tokyo). 1978 May;31(5):421-5 PMID: 353012
  28. Globomycin, a new peptide antibiotic with spheroplast-forming activity. III. Structural determination of globomycin.
    J Antibiot (Tokyo). 1978 May;31(5):426-32 PMID: 670085
  29. Accumulation of glyceride-containing precursor of the outer membrane lipoprotein in the cytoplasmic membrane of Escherichia coli treated with globomycin.
    J Biol Chem. 1980 Apr 25;255(8):3707-12 PMID: 6988430
  30. Lipoproteins of Haemophilus influenzae type b.
    J Bacteriol. 1988 Sep;170(9):4161-4 PMID: 3261724
  31. Characterization of new membrane lipoproteins and their precursors of Escherichia coli.
    J Biol Chem. 1981 Mar 25;256(6):3125-9 PMID: 7009608
  32. Synthesis of a precursor to the B subunit of heat-labile enterotoxin in Escherichia coli.
    J Bacteriol. 1981 Apr;146(1):325-30 PMID: 6260742
  33. Construction and characterization of new cloning vehicles. V. Mobilization and coding properties of pBR322 and several deletion derivatives including pBR327 and pBR328.
    Gene. 1981 Jan-Feb;13(1):25-35 PMID: 6263753
  34. Lipoprotein nature of Bacillus licheniformis membrane penicillinase.
    Proc Natl Acad Sci U S A. 1981 Jun;78(6):3511-5 PMID: 7022453
  35. Murine monoclonal antibodies specific for virulent Treponema pallidum (Nichols).
    Infect Immun. 1982 Jun;36(3):1076-85 PMID: 7047388
  36. Post-translational modification and processing of Escherichia coli prolipoprotein in vitro.
    Proc Natl Acad Sci U S A. 1982 Apr;79(7):2255-9 PMID: 7048314
  37. Molecular cloning and expression of Treponema pallidum DNA in Escherichia coli K-12.
    Infect Immun. 1983 Oct;42(1):187-96 PMID: 6311744
  38. Cloning and expression of Treponema pallidum (Nichols) antigen genes in Escherichia coli.
    Infect Immun. 1983 Nov;42(2):435-45 PMID: 6358023
  39. Prolipoprotein modification and processing enzymes in Escherichia coli.
    J Biol Chem. 1984 Mar 25;259(6):3825-30 PMID: 6368552
  40. Monoclonal antibody with hemagglutination, immobilization, and neutralization activities defines an immunodominant, 47,000 mol wt, surface-exposed immunogen of Treponema pallidum (Nichols).
    J Exp Med. 1984 Nov 1;160(5):1404-20 PMID: 6208310
  41. Nucleotide sequence of pilA, the gene encoding the structural component of type 1 pili in Escherichia coli.
    J Bacteriol. 1985 Apr;162(1):454-7 PMID: 2858471
  42. Genetic characterization and partial sequence determination of a Treponema pallidum operon expressing two immunogenic membrane proteins in Escherichia coli.
    J Bacteriol. 1985 Jun;162(3):1227-37 PMID: 3922944
  43. Inhibition of prolipoprotein signal peptidase by globomycin.
    J Biol Chem. 1985 May 25;260(10):5891-4 PMID: 3888977
  44. DNA sequence of the Serratia marcescens lipoprotein gene.
    Proc Natl Acad Sci U S A. 1980 Mar;77(3):1369-73 PMID: 6990409
Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.58.2.384-392.1990
Published
1990-02-00
Pages
384-92
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC258467
Subset
IM
Grants
NIAID NIH HHS · AI-16692 · United States
NIAID NIH HHS · AI-17366 · United States
NIAID NIH HHS · AI-26756 · United States
External Links
PubMed source DOI: 10.1128/iai.58.2.384-392.1990 PMC Full Text View journal details
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