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PMID: 3118373 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Myristoylation and the post-translational acquisition of hydrophobicity by the membrane immunoglobulin heavy-chain polypeptide in B lymphocytes.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 84 ·No. 21 ·1987-11-00 ·Pages 7654-8

Pillai S, Baltimore D

Abstract

Membrane immunoglobulin heavy chain in pre-B and in B cells is initially synthesized as a relatively hydrophilic protein that is nonetheless stably anchored in the endoplasmic reticulum membrane. In B cells, but not in pre-B cells, the membrane immunoglobulin heavy chain is post-translationally converted to a relatively hydrophobic form that partitions into the oil phase when solubilized with the phase-separating detergent Triton X-114. Covalent myristoylation of the membrane and secretory forms of immunoglobulin heavy chains as well as of light chains was observed in B cells. Myristoylation of the membrane immunoglobulin heavy chain correlates with its transport to the cell surface and its post-translational conversion to a relatively hydrophobic form. This post-translational modification is hydroxylamine resistant and may be responsible for the assembly and transport of membrane immunoglobulin to the cell surface in B cells.

MeSH Terms
Animals B-Lymphocytes/immunology Cell Line Immunoglobulin Heavy Chains/genetics Immunoglobulin Light Chains/genetics Immunoglobulin mu-Chains/genetics Kinetics Mice Myristic Acids/metabolism Protein Processing, Post-Translational
Chemicals
Immunoglobulin Heavy Chains Immunoglobulin Light Chains Immunoglobulin mu-Chains Myristic Acids
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Pillai S
Whitehead Institute for Biomedical Research, Cambridge, MA 02142.
Baltimore D
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.84.21.7654
Published
1987-11-00
Pages
7654-8
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC299358
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.84.21.7654 PMC Full Text View journal details
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