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PMID: 2403645 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

The sum1-1 mutation affects silent mating-type gene transcription in Saccharomyces cerevisiae.

Molecular and cellular biology ·Vol. 10 ·No. 1 ·1990-01-00 ·Pages 409-12

Livi GP, Hicks JB, Klar AJ

Abstract

The silent mating-type genes (HML and HMR) of Saccharomyces cerevisiae are kept under negative transcriptional control by the trans-acting products of the four MAR/SIR loci. MAR/SIR gene mutations result in the simultaneous derepression of HML and HMR gene expression. The sum1-1 mutation was previously identified as an extragenic suppressor of mutations in MAR1 (SIR2) and MAR2 (SIR3). As assayed genetically, sum1-1 is capable of restoring repression of silent mating-type information in cells containing mar1 or mar2 null mutations. We show here that the mating-type phenotype associated with sum1-1 results from a dramatic reduction in the steady-state level of HML and HMR gene transcripts. At the same time, the sum1-1 mutation has no significant effect on the level of each of the four MAR/SIR mRNAs.

MeSH Terms
Blotting, Northern Gene Expression Regulation, Fungal Genes, Fungal RNA, Fungal/genetics RNA, Messenger/genetics Saccharomyces cerevisiae/genetics Transcription, Genetic
Chemicals
RNA, Fungal RNA, Messenger
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Livi G P
Cold Spring Harbor Laboratory, New York 11724.
Hicks J B
Klar A J
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36 references, click to expand
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.10.1.409-412.1990
Published
1990-01-00
Pages
409-12
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC360769
Subset
IM
Grants
NIGMS NIH HHS · GM25678 · United States
External Links
PubMed source DOI: 10.1128/mcb.10.1.409-412.1990 PMC Full Text View journal details
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