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PMID: 2395867 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Simultaneous localization of cosmids and chromosome R-banding by fluorescence microscopy: application to regional mapping of human chromosome 11.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 87 ·No. 17 ·1990-09-00 ·Pages 6639-43

Cherif D, Julier C, Delattre O, Derré J, Lathrop GM, Berger R

Abstract

A technique for nonradioactive in situ hybridization on human metaphase chromosomes has been developed to localize human cosmid clones. The simple procedure using two fluorescent dyes (fluorescein and propidium iodide) allows the simultaneous identification of chromosomal R-bands and hybridization signal in a single screening of the slides. This technique has been used for rapid correlation of the genetic and physical map of chromosome 11q13-qter in the region of genes responsible for ataxia-telangiectasia and tuberous sclerosis.

MeSH Terms
Cell Line Chromosome Banding Chromosome Mapping Chromosomes, Human, Pair 11 Clone Cells Cosmids Genetic Linkage Humans Hybrid Cells/cytology Microscopy, Fluorescence/methods Nucleic Acid Hybridization
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Cherif D
Institut National de la Santé et de la Recherche Médicale, Unité 301 et Structure d'Intervention No. 15954 Centre National de la Recherche Scientifique, Paris, France.
Julier C
Delattre O
Derré J
Lathrop G M
Berger R
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.87.17.6639
Published
1990-09-00
Pages
6639-43
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC54592
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.87.17.6639 PMC Full Text View journal details
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