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PMID: 3461479 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Comparison of constitutional and tumor-associated 11;22 translocations: nonidentical breakpoints on chromosomes 11 and 22.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 83 ·No. 16 ·1986-08-00 ·Pages 6122-6

Griffin CA, McKeon C, Israel MA, Gegonne A, Ghysdael J, Stehelin D, Douglass EC, Green AE, Emanuel BS

Abstract

Recurring, site-specific chromosomal rearrangements are associated with several human syndromes and malignant disorders. Such nonrandom translocations involving chromosome 22 in band q11 are numerous and found to be associated with a diversity of neoplasms as well as constitutional disorders. Chromosome 11 in bands q23-q24 is similarly involved in several types of tumors as well as in a recurring constitutional reciprocal translocation with chromosome 22. Here we report the use of chromosomal in situ hybridization to compare the translocation breakpoints in the cytologically indistinguishable constitutional t(11;22) and the tumor-related t(11;22) associated with Ewing sarcoma and peripheral neuroepithelioma. We have shown that the breakpoints can be distinguished from each other with respect to the locus encoding the constant region of the Ig lambda light chain (C lambda) at 22q11 and the ETS1 locus at 11q23----q24; ETS1 has been called hu-ets-1 or human c-ets-1. The tumor-associated chromosome 11 breakpoint is also different from those of leukemias with t(9;11) and t(4;11) translocations. Southern-blot analysis showed no rearrangement of ETS1 in these disorders in the region detected by our probe. ETS1 has also been mapped more precisely to 11q23.3----q24 by in situ hybridization to cells from an individual with an 11q23.3----qter deletion.

MeSH Terms
Cell Line Chromosome Mapping Chromosomes, Human, 21-22 and Y Chromosomes, Human, 6-12 and X Female Humans Karyotyping Leukemia/genetics Male Metaphase Nucleic Acid Hybridization Translocation, Genetic
Authors & Affiliations
9 authors, click to expand affiliations / ORCID
Griffin C A
McKeon C
Israel M A
Gegonne A
Ghysdael J
Stehelin D
Douglass E C
Green A E
Emanuel B S
References (36)
36 references, click to expand
  1. Letter: A new consistent chromosomal abnormality in chronic myelogenous leukaemia identified by quinacrine fluorescence and Giemsa staining.
    Nature. 1973 Jun 1;243(5405):290-3 PMID: 4126434
  2. An 8-kilobase abl RNA transcript in chronic myelogenous leukemia.
    Proc Natl Acad Sci U S A. 1984 Sep;81(18):5648-52 PMID: 6091099
  3. Detection of specific sequences among DNA fragments separated by gel electrophoresis.
    J Mol Biol. 1975 Nov 5;98(3):503-17 PMID: 1195397
  4. Quantitative analysis of high-resolution trypsin-giemsa bands on human prometaphase chromosomes.
    Hum Genet. 1978 Dec 18;45(2):137-65 PMID: 738718
  5. Site-specific reciprocal translocation, t(11;22) (q23;q11), in several unrelated families with 3:1 meiotic disjunction.
    Am J Med Genet. 1980;7(4):507-21 PMID: 7211960
  6. Assignment of the genes for human lambda immunoglobulin chains to chromosome 22.
    Nature. 1981 Nov 12;294(5837):173-5 PMID: 6795508
  7. Unusual methylation pattern of the alpha 2 (l) collagen gene.
    Cell. 1982 May;29(1):203-10 PMID: 7105182
  8. Neuroblastoma and the differential diagnosis of small-, round-, blue-cell tumors.
    Hum Pathol. 1983 Jul;14(7):569-95 PMID: 6345338
  9. The 11q;22q translocation: a collaborative study of 20 new cases and analysis of 110 families.
    Hum Genet. 1983;64(4):343-55 PMID: 6618487
  10. Reciprocal translocation in small-cell sarcomas.
    N Engl J Med. 1984 Dec 27;311(26):1702-3 PMID: 6504115
  11. An improved method for G-banding chromosomes after in situ hybridization.
    Cytogenet Cell Genet. 1984;38(4):308-9 PMID: 6439479
  12. Translocation of oncogene c-sis from chromosome 22 to chromosome 11 in a Ewing sarcoma-derived cell line.
    Mol Cell Biol. 1985 Feb;5(2):427-9 PMID: 2983195
  13. Assignment of the gene coding for the T3-delta subunit of the T3-T-cell receptor complex to the long arm of human chromosome 11 and to mouse chromosome 9.
    Proc Natl Acad Sci U S A. 1985 May;82(9):2920-4 PMID: 3857625
  14. Clustering of breakpoints on chromosome 11 in human B-cell neoplasms with the t(11;14) chromosome translocation.
    Nature. 1985 May 23-29;315(6017):340-3 PMID: 3923362
  15. Human alpha 1(III) and alpha 2(V) procollagen genes are located on the long arm of chromosome 2.
    Proc Natl Acad Sci U S A. 1985 May;82(10):3385-9 PMID: 3858826
  16. Fused transcript of abl and bcr genes in chronic myelogenous leukaemia.
    Nature. 1985 Jun 13-19;315(6020):550-4 PMID: 2989692
  17. Structural organization of the bcr gene and its role in the Ph' translocation.
    Nature. 1985 Jun 27-Jul 3;315(6022):758-61 PMID: 2989703
  18. Translocation breakpoint mapping: molecular and cytogenetic studies of chromosome 22.
    Cancer Genet Cytogenet. 1986 Jan 1;19(1-2):81-92 PMID: 3455663
  19. Interferon and c-ets-1 genes in the translocation (9;11)(p22;q23) in human acute monocytic leukemia.
    Science. 1986 Jan 17;231(4735):265-7 PMID: 3455787
  20. Hu-ets-1 and Hu-ets-2 genes are transposed in acute leukemias with (4;11) and (8;21) translocations.
    Science. 1986 Jan 24;231(4736):379-82 PMID: 3941901
  21. Cytogenetic characterization of selected small round cell tumors of childhood.
    Cancer Genet Cytogenet. 1986 Apr 1;21(3):185-208 PMID: 3004699
  22. Heterogeneity of chromosome 22 breakpoint in Philadelphia-positive (Ph+) acute lymphocytic leukemia.
    Proc Natl Acad Sci U S A. 1986 Mar;83(6):1807-11 PMID: 3513189
  23. Characterization of the supernumerary chromosome in cat eye syndrome.
    Science. 1986 May 2;232(4750):646-8 PMID: 3961499
  24. Transcriptional activation of an unrearranged and untranslocated c-myc oncogene by translocation of a C lambda locus in Burkitt.
    Proc Natl Acad Sci U S A. 1983 Nov;80(22):6922-6 PMID: 6417658
  25. The c-sis oncogene is not activated in Ewing's sarcoma.
    N Engl J Med. 1984 Feb 9;310(6):393 PMID: 6690975
  26. Philadelphia chromosomal breakpoints are clustered within a limited region, bcr, on chromosome 22.
    Cell. 1984 Jan;36(1):93-9 PMID: 6319012
  27. Localization of the cellular oncogenes ABL, SIS, and FES on human germ-line chromosomes.
    Cytogenet Cell Genet. 1984;38(1):73-5 PMID: 6323103
  28. Chromosome study of Ewing's sarcoma (ES) cell lines. Consistency of a reciprocal translocation t(11;22)(q24;q12).
    Cancer Genet Cytogenet. 1984 May;12(1):1-19 PMID: 6713356
  29. Translocation involving chromosome 22 in Ewing's sarcoma. A cytogenetic study of four fresh tumors.
    Cancer Genet Cytogenet. 1984 May;12(1):21-5 PMID: 6713357
  30. Altered transcription of the c-abl oncogene in K-562 and other chronic myelogenous leukemia cells.
    Science. 1984 Jul 6;225(4657):72-4 PMID: 6587568
  31. An alteration of the human c-abl protein in K562 leukemia cells unmasks associated tyrosine kinase activity.
    Cell. 1984 Jul;37(3):1035-42 PMID: 6204766
  32. Chromosome translocation in peripheral neuroepithelioma.
    N Engl J Med. 1984 Aug 30;311(9):584-5 PMID: 6749231
  33. Chromosomal localization of the human proto-oncogene c-ets.
    Nature. 1984 Aug 16-22;310(5978):581-3 PMID: 6462246
  34. In situ hybridization and translocation breakpoint mapping. I. Nonidentical 22q11 breakpoints for the t(9;22) of CML and the t(8;22) of Burkitt lymphoma.
    Cytogenet Cell Genet. 1984;38(2):127-31 PMID: 6467987
  35. Chromosomal abnormalities in secondary Ewing's sarcoma.
    Lancet. 1984 Oct 6;2(8406):812 PMID: 6148551
  36. Analysis of repeating DNA sequences by reassociation.
    Methods Enzymol. 1974;29:363-418 PMID: 4850571
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.83.16.6122
Published
1986-08-00
Pages
6122-6
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC386451
Subset
IM
Grants
NCI NIH HHS · CA 09485 · United States
NCI NIH HHS · CA 39926 · United States
NIGMS NIH HHS · GM 32592 · United States
External Links
PubMed source DOI: 10.1073/pnas.83.16.6122 PMC Full Text View journal details
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