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PMID: 2388824 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Distortions induced in double-stranded oligonucleotides by the binding of cis- or trans-diammine-dichloroplatinum(II) to the d(GTG) sequence.

Nucleic acids research ·Vol. 18 ·No. 15 ·1990-08-11 ·Pages 4395-400

Anin MF, Leng M

Abstract

Conformational changes induced in double-stranded oligonucleotides by the binding of trans- or cis-diamminedichloro platinum(II) to the d(GTG) sequence have been characterized by means of melting temperatures, electrophoretic migrations in non-denaturing polyacrylamide gels, reactivities with the artificial nuclease Phenanthroline-copper and with chemical probes. The cis-platinum adduct behaves more as a centre of directed bend than as a hinge joint, the induced bend angle being of the order of 25-30 degrees. The double helix is locally denatured over 2 base pairs (corresponding to the platinated 5'G residue and the central T residue) and is distorted over 4-5 base pairs. The trans-platinum adduct behaves also more as a centre of directed bend than as a hinge joint, the induced bend angle being of the order of 60 degrees. The double helix is locally denatured over 4 base pairs (corresponding to the immediately 5'T residue adjacent to the adduct and to the three base residues of the adduct). Both the cis- and trans-platinum adducts decrease the thermal stability of the double helix.

MeSH Terms
Acetaldehyde/analogs & derivatives Base Sequence Binding Sites Cisplatin/metabolism Copper Diethyl Pyrocarbonate Electrophoresis, Gel, Two-Dimensional Isomerism Molecular Sequence Data Nucleic Acid Conformation Oligodeoxyribonucleotides Osmium Tetroxide Phenanthrolines Sodium Cyanide Temperature
Chemicals
Oligodeoxyribonucleotides Phenanthrolines transplatin Copper chloroacetaldehyde Acetaldehyde Diethyl Pyrocarbonate Sodium Cyanide Osmium Tetroxide Cisplatin
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Anin M F
Centre de Biophysique Moléculaire, CNRS, Orléans, France.
Leng M
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28 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/18.15.4395
Published
1990-08-11
Pages
4395-400
Language
English
Region
England
NLM ID
0411011
PMCID
PMC331256
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/18.15.4395 PMC Full Text View journal details
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