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PMID: 2377607 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Gene expression of fibroblast growth factors in human gliomas and meningiomas: demonstration of cellular source of basic fibroblast growth factor mRNA and peptide in tumor tissues.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 87 ·No. 15 ·1990-08-00 ·Pages 5710-4

Takahashi JA, Mori H, Fukumoto M, Igarashi K, Jaye M, Oda Y, Kikuchi H, Hatanaka M

Abstract

The growth autonomy of human tumor cells is considered due to the endogenous production of growth factors. Transcriptional expression of candidates for autocrine stimulatory factors such as basic fibroblast growth factor (FGF), acidic FGF, and transforming growth factor type beta were determined in human brain tumors. Basic FGF was expressed abundantly in 17 of 18 gliomas, 20 of 22 meninglomas, and 0 of 5 metastatic brain tumors. The level of mRNA expression of acidic FGF in gliomas was significant. In contrast, transforming growth factor type beta 1 was expressed in all the samples investigated. The mRNA for basic FGF and its peptide were localized in tumor cells in vivo by in situ hybridization and immunohistochemistry, showing that basic FGF is actually produced in tumor cells. Our results suggest that tumor-derived basic FGF is involved in the progression of gliomas and meningiomas in vivo, whereas acidic FGF is expressed in a tumor origin-specific manner, suggesting that acidic FGF works in tandem with basic FGF in glioma tumorigenesis.

MeSH Terms
Blotting, Northern Brain Neoplasms/genetics,metabolism Fibroblast Growth Factors/biosynthesis,genetics,isolation & purification Gene Expression Glioma/genetics,metabolism Humans Meningeal Neoplasms/genetics,metabolism Meningioma/genetics,metabolism Nucleic Acid Hybridization RNA, Messenger/genetics,isolation & purification RNA, Neoplasm/genetics,isolation & purification
Chemicals
RNA, Messenger RNA, Neoplasm Fibroblast Growth Factors
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Takahashi J A
Department of Molecular Virology, Faculty of Medicine, Kyoto University, Japan.
Mori H
Fukumoto M
Igarashi K
Jaye M
Oda Y
Kikuchi H
Hatanaka M
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.87.15.5710
Published
1990-08-00
Pages
5710-4
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC54397
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.87.15.5710 PMC Full Text View journal details
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