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PMID: 23757404 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Export of salicylic acid from the chloroplast requires the multidrug and toxin extrusion-like transporter EDS5.

Plant physiology ·Vol. 162 ·No. 4 ·2013-08-00 ·Pages 1815-21

Serrano M, Wang B, Aryal B, Garcion C, Abou-Mansour E, Heck S, Geisler M, Mauch F, Nawrath C, Métraux JP

Abstract

Salicylic acid (SA) is central for the defense of plants to pathogens and abiotic stress. SA is synthesized in chloroplasts from chorismic acid by an isochorismate synthase (ICS1); SA biosynthesis is negatively regulated by autoinhibitory feedback at ICS1. Genetic studies indicated that the multidrug and toxin extrusion transporter ENHANCED DISEASE SUSCEPTIBILITY5 (EDS5) of Arabidopsis (Arabidopsis thaliana) is necessary for SA accumulation after biotic and abiotic stress, but so far it is not understood how EDS5 controls the biosynthesis of SA. Here, we show that EDS5 colocalizes with a marker of the chloroplast envelope and that EDS5 functions as a multidrug and toxin extrusion-like transporter in the export of SA from the chloroplast to the cytoplasm in Arabidopsis, where it controls the innate immune response. The location at the chloroplast envelope supports a model of the effect of EDS5 on SA biosynthesis: in the eds5 mutant, stress-induced SA is trapped in the chloroplast and inhibits its own accumulation by autoinhibitory feedback.

MeSH Terms
Arabidopsis/drug effects,physiology,radiation effects Arabidopsis Proteins/genetics,metabolism Biological Transport Carbonyl Cyanide m-Chlorophenyl Hydrazone/pharmacology Chloroplasts/genetics,metabolism Cytoplasm/genetics,metabolism Immunity, Innate Membrane Transport Proteins/genetics,metabolism Molecular Sequence Data Mutation Nigericin/pharmacology Plants, Genetically Modified Protoplasts/metabolism Salicylic Acid/metabolism Ultraviolet Rays
Chemicals
Arabidopsis Proteins EDS5 protein, Arabidopsis Membrane Transport Proteins Carbonyl Cyanide m-Chlorophenyl Hydrazone Salicylic Acid Nigericin
Authors & Affiliations
10 authors, click to expand affiliations / ORCID
Serrano Mario
Department of Biology, University of Fribourg, 1700 Fribourg, Switzerland.
Wang Bangjun
Aryal Bibek
Garcion Christophe
Abou-Mansour Eliane
Heck Silvia
Geisler Markus
Mauch Felix
Nawrath Christiane
Métraux Jean-Pierre
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Article Info
Journal
Plant physiology
Abbr.
Plant Physiol
ISSN
1532-2548
DOI
10.1104/pp.113.218156
Published
2013-08-00
Epub
2013-00-11
Pages
1815-21
Language
English
Region
United States
NLM ID
0401224
PMCID
PMC3729763
Subset
IM
Databases
GENBANK
AF416569
External Links
PubMed source DOI: 10.1104/pp.113.218156 PMC Full Text View journal details
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