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PMID: 11826312 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

EDS5, an essential component of salicylic acid-dependent signaling for disease resistance in Arabidopsis, is a member of the MATE transporter family.

The Plant cell ·Vol. 14 ·No. 1 ·2002-01-00 ·Pages 275-86

Nawrath C, Heck S, Parinthawong N, Métraux JP

Abstract

The eds5 mutant of Arabidopsis (earlier named sid1) was shown previously to accumulate very little salicylic acid and PR-1 transcript after pathogen inoculation and to be hypersusceptible to pathogens. We have isolated EDS5 by positional cloning and show that it encodes a protein with a predicted series of nine to 11 membrane-spanning domains and a coil domain at the N terminus. EDS5 is homologous with members of the MATE (multidrug and toxin extrusion) transporter family. EDS5 expression is very low in unstressed plants and strongly induced by pathogens and UV-C light. The transcript starts to accumulate 2 hr after inoculation of Arabidopsis with an avirulent strain of Pseudomonas syringae or UV-C light exposure, and it stays induced for approximately 2 days. EDS5 also is expressed after treatments with salicylic acid, indicating a possible positive feedback regulation. EDS5 expression after infection by certain pathogens as well as after UV-C light exposure depends on the pathogen response proteins EDS1, PAD4, and NDR1, indicating that the signal transduction pathways after UV-C light exposure and pathogen inoculation share common elements.

MeSH Terms
Amino Acid Sequence Arabidopsis/drug effects,genetics,radiation effects Arabidopsis Proteins/genetics,radiation effects Base Sequence Biological Transport Carboxylic Ester Hydrolases/genetics Chromosome Mapping Cloning, Molecular DNA, Complementary/chemistry,genetics DNA-Binding Proteins/genetics Gene Expression Regulation, Plant/drug effects,radiation effects Immunity, Innate/genetics Membrane Proteins/genetics Membrane Transport Proteins/genetics,radiation effects Molecular Sequence Data Mutation Plant Diseases/genetics,microbiology Pseudomonas/growth & development Salicylic Acid/pharmacology Sequence Alignment Sequence Analysis, DNA Sequence Homology, Amino Acid Signal Transduction/drug effects,radiation effects Transcription Factors/genetics Ultraviolet Rays
Chemicals
Arabidopsis Proteins DNA, Complementary DNA-Binding Proteins EDS1 protein, Arabidopsis EDS5 protein, Arabidopsis Membrane Proteins Membrane Transport Proteins NDR1 protein, Arabidopsis Transcription Factors Carboxylic Ester Hydrolases PAD4 protein, Arabidopsis Salicylic Acid
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Nawrath Christiane
Department of Biology, Unit of Plant Biology, Route Albert Gockel 3, 1700 Fribourg, Switzerland. christiane.nawrath@unifr.ch
Heck Silvia
Parinthawong Nonglak
Métraux Jean-Pierre
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Article Info
Journal
The Plant cell
Abbr.
Plant Cell
ISSN
1040-4651
DOI
10.1105/tpc.010376
Email
christiane.nawrath@unifr.ch
Published
2002-01-00
Pages
275-86
Language
English
Region
England
NLM ID
9208688
PMCID
PMC150564
Subset
IM
Databases
GENBANK
AF416569
External Links
PubMed source DOI: 10.1105/tpc.010376 PMC Full Text View journal details
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