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PMID: 23661758 Published · ppublish English Journal Article Research Support, N.I.H., Extramural

Networks of bZIP protein-protein interactions diversified over a billion years of evolution.

Science (New York, N.Y.) ·Vol. 340 ·No. 6133 ·2013-05-10 ·Pages 730-4

Reinke AW, Baek J, Ashenberg O, Keating AE

Abstract

Differences in biomolecular sequence and function underlie dramatic ranges of appearance and behavior among species. We studied the basic region-leucine zipper (bZIP) transcription factors and quantified bZIP dimerization networks for five metazoan and two single-cell species, measuring interactions in vitro for 2891 protein pairs. Metazoans have a higher proportion of heteromeric bZIP interactions and more network complexity than the single-cell species. The metazoan bZIP interactomes have broadly similar structures, but there has been extensive rewiring of connections compared to the last common ancestor, and each species network is highly distinct. Many metazoan bZIP orthologs and paralogs have strikingly different interaction specificities, and some differences arise from minor sequence changes. Our data show that a shifting landscape of biochemical functions related to signaling and gene expression contributes to species diversity.

MeSH Terms
Amino Acid Sequence Animals Basic-Leucine Zipper Transcription Factors/chemistry,genetics,metabolism Conserved Sequence Evolution, Molecular Humans Metabolic Networks and Pathways Molecular Sequence Data Protein Multimerization
Chemicals
Basic-Leucine Zipper Transcription Factors
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Reinke Aaron W
Massachusetts Institute of Technology, Department of Biology, Cambridge, MA 02139, USA.
Baek Jiyeon
Ashenberg Orr
Keating Amy E
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Article Info
Journal
Science (New York, N.Y.)
Abbr.
Science
ISSN
1095-9203
DOI
10.1126/science.1233465
Published
2013-05-10
Pages
730-4
Language
English
Region
United States
NLM ID
0404511
PMCID
PMC4115154
Subset
IM
Grants
NIGMS NIH HHS · R01 GM067681 · United States
NIGMS NIH HHS · GM067681 · United States
External Links
PubMed source DOI: 10.1126/science.1233465 PMC Full Text View journal details
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