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PMID: 23348738 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Platelet ITAM signaling is critical for vascular integrity in inflammation.

The Journal of clinical investigation ·Vol. 123 ·No. 2 ·2013-02-00 ·Pages 908-16

Boulaftali Y, Hess PR, Getz TM, Cholka A, Stolla M, Mackman N, Owens AP, Ware J, Kahn ML, Bergmeier W

Abstract

Platelets play a critical role in maintaining vascular integrity during inflammation, but little is known about the underlying molecular mechanisms. Here we report that platelet immunoreceptor tyrosine activation motif (ITAM) signaling, but not GPCR signaling, is critical for the prevention of inflammation-induced hemorrhage. To generate mice with partial or complete defects in these signaling pathways, we developed a protocol for adoptive transfer of genetically and/or chemically inhibited platelets into thrombocytopenic (TP) mice. Unexpectedly, platelets with impaired GPCR signaling, a crucial component of platelet plug formation and hemostasis, were indistinguishable from WT platelets in their ability to prevent hemorrhage at sites of inflammation. In contrast, inhibition of GPVI or genetic deletion of Clec2, the only ITAM receptors expressed on mouse platelets, significantly reduced the ability of platelets to prevent inflammation-induced hemorrhage. Moreover, transfusion of platelets without ITAM receptor function or platelets lacking the adapter protein SLP-76 into TP mice had no significant effect on vascular integrity during inflammation. These results indicate that the control of vascular integrity is a major function of immune-type receptors in platelets, highlighting a potential clinical complication of novel antithrombotic agents directed toward the ITAM signaling pathway.

MeSH Terms
Adaptor Proteins, Signal Transducing/antagonists & inhibitors,blood Adoptive Transfer Animals Blood Platelets/physiology Blood Vessels/physiopathology Hemostasis Inflammation/blood,physiopathology Lectins, C-Type/deficiency,genetics Male Mice Mice, Inbred C57BL Mice, Knockout Mice, Transgenic Phosphoproteins/antagonists & inhibitors,blood Platelet Membrane Glycoproteins/antagonists & inhibitors,physiology Receptors, G-Protein-Coupled/blood,physiology Receptors, Thrombin/blood,deficiency,genetics Signal Transduction Thrombocytopenia/blood,physiopathology
Chemicals
Adaptor Proteins, Signal Transducing CLEC-2 protein, mouse Lectins, C-Type Phosphoproteins Platelet Membrane Glycoproteins Receptors, G-Protein-Coupled Receptors, Thrombin SLP-76 signal Transducing adaptor proteins platelet membrane glycoprotein VI protease-activated receptor 4
Authors & Affiliations
10 authors, click to expand affiliations / ORCID
Boulaftali Yacine
McAllister Heart Institute, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA.
Hess Paul R
Getz Todd M
Cholka Agnieszka
Stolla Moritz
Mackman Nigel
Owens A Phillip
Ware Jerry
Kahn Mark L
Bergmeier Wolfgang
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Article Info
Journal
The Journal of clinical investigation
Abbr.
J Clin Invest
ISSN
1558-8238
Published
2013-02-00
Epub
2013-00-25
Pages
908-16
Language
English
Region
United States
NLM ID
7802877
PMCID
PMC3561801
Subset
IM
Grants
NHLBI NIH HHS · R01 HL050545 · United States
NHLBI NIH HHS · R01 HL094594 · United States
NCI NIH HHS · P30 CA016086 · United States
NHLBI NIH HHS · T32 HL007149 · United States
NHLBI NIH HHS · HL106009 · United States
NHLBI NIH HHS · R01 HL103432 · United States
NHLBI NIH HHS · P01 HL006350 · United States
NHLBI NIH HHS · F32 HL099175 · United States
NHLBI NIH HHS · HL006350 · United States
NHLBI NIH HHS · HL072798 · United States
NHLBI NIH HHS · R01 HL067311 · United States
NHLBI NIH HHS · R01 HL072798 · United States
NHLBI NIH HHS · R01 HL106009 · United States
NHLBI NIH HHS · HL50545 · United States
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