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PMID: 23344721 Published · epublish English Journal Article

miR-1289 and "Zipcode"-like Sequence Enrich mRNAs in Microvesicles.

Molecular therapy. Nucleic acids ·Vol. 1 ·2012-02-07 ·Pages e10

Bolukbasi MF, Mizrak A, Ozdener GB, Madlener S, Ströbel T, Erkan EP, Fan JB, Breakefield XO, Saydam O

Abstract

Despite intensive studies, the molecular mechanisms by which the genetic materials are uploaded into microvesicles (MVs) are still unknown. This is the first study describing a zipcode-like 25 nucleotide (nt) sequence in the 3'-untranslated region (3'UTR) of mRNAs, with variants of this sequence present in many mRNAs enriched in MVs, as compared to their glioblastoma cells of origin. When this sequence was incorporated into the 3'UTR of a reporter message and expressed in a different cell type, it led to enrichment of the reporter mRNA in MVs. Critical features of this sequence are both a CUGCC core presented on a stem-loop structure and a miRNA-binding site, with increased levels of the corresponding miRNA in cells further increasing levels of mRNAs in MVs.

Authors & Affiliations
9 authors, click to expand affiliations / ORCID
Bolukbasi Mehmet Fatih
Department of Neurology and Radiology, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, USA.
Mizrak Arda
Ozdener Gokhan Baris
Madlener Sibylle
Ströbel Thomas
Erkan Erdogan Pekcan
Fan Jian-Bing
Breakefield Xandra O
Saydam Okay
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Article Info
Journal
Molecular therapy. Nucleic acids
Abbr.
Mol Ther Nucleic Acids
ISSN
2162-2531
DOI
10.1038/mtna.2011.2
Published
2012-02-07
Epub
2012-00-07
Pages
e10
Language
English
Region
United States
NLM ID
101581621
PMCID
PMC3381601
Grants
NINDS NIH HHS · P01 NS037409 · United States
NCI NIH HHS · R01 CA141150 · United States
External Links
PubMed source DOI: 10.1038/mtna.2011.2 PMC Full Text View journal details
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