Abstract
To realize the therapeutic potential of RNA drugs, efficient, tissue-specific and nonimmunogenic delivery technologies must be developed. Here we show that exosomes-endogenous nano-vesicles that transport RNAs and proteins-can deliver short interfering (si)RNA to the brain in mice. To reduce immunogenicity, we used self-derived dendritic cells for exosome production. Targeting was achieved by engineering the dendritic cells to express Lamp2b, an exosomal membrane protein, fused to the neuron-specific RVG peptide. Purified exosomes were loaded with exogenous siRNA by electroporation. Intravenously injected RVG-targeted exosomes delivered GAPDH siRNA specifically to neurons, microglia, oligodendrocytes in the brain, resulting in a specific gene knockdown. Pre-exposure to RVG exosomes did not attenuate knockdown, and non-specific uptake in other tissues was not observed. The therapeutic potential of exosome-mediated siRNA delivery was demonstrated by the strong mRNA (60%) and protein (62%) knockdown of BACE1, a therapeutic target in Alzheimer's disease, in wild-type mice.
MeSH Terms
Alzheimer Disease/therapy
Amyloid Precursor Protein Secretases/genetics
Animals
Aspartic Acid Endopeptidases/genetics
Brain/metabolism
Cell Line
Dendritic Cells/metabolism
Exosomes/metabolism
Gene Knockdown Techniques
Gene Transfer Techniques
Genetic Vectors
Lysosomal-Associated Membrane Protein 2/genetics
Male
Membrane Proteins/metabolism
Mice
Mice, Inbred C57BL
Microglia/metabolism
Neurons/metabolism
Peptides/metabolism
Proteins/metabolism
RNA, Messenger/metabolism
RNA, Small Interfering/administration & dosage
Rabies virus/metabolism
Chemicals
Lysosomal-Associated Membrane Protein 2
Membrane Proteins
Peptides
Proteins
RNA, Messenger
RNA, Small Interfering
Amyloid Precursor Protein Secretases
Aspartic Acid Endopeptidases
Bace1 protein, mouse
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Alvarez-Erviti Lydia
Department of Physiology, Anatomy and Genetics, University of Oxford, Oxford, UK.
Seow Yiqi
Yin Haifang
Betts Corinne
Lakhal Samira
Wood Matthew J A
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