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PMID: 21423189 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Delivery of siRNA to the mouse brain by systemic injection of targeted exosomes.

Nature biotechnology ·Vol. 29 ·No. 4 ·2011-04-00 ·Pages 341-5

Alvarez-Erviti L, Seow Y, Yin H, Betts C, Lakhal S, Wood MJ

Abstract

To realize the therapeutic potential of RNA drugs, efficient, tissue-specific and nonimmunogenic delivery technologies must be developed. Here we show that exosomes-endogenous nano-vesicles that transport RNAs and proteins-can deliver short interfering (si)RNA to the brain in mice. To reduce immunogenicity, we used self-derived dendritic cells for exosome production. Targeting was achieved by engineering the dendritic cells to express Lamp2b, an exosomal membrane protein, fused to the neuron-specific RVG peptide. Purified exosomes were loaded with exogenous siRNA by electroporation. Intravenously injected RVG-targeted exosomes delivered GAPDH siRNA specifically to neurons, microglia, oligodendrocytes in the brain, resulting in a specific gene knockdown. Pre-exposure to RVG exosomes did not attenuate knockdown, and non-specific uptake in other tissues was not observed. The therapeutic potential of exosome-mediated siRNA delivery was demonstrated by the strong mRNA (60%) and protein (62%) knockdown of BACE1, a therapeutic target in Alzheimer's disease, in wild-type mice.

MeSH Terms
Alzheimer Disease/therapy Amyloid Precursor Protein Secretases/genetics Animals Aspartic Acid Endopeptidases/genetics Brain/metabolism Cell Line Dendritic Cells/metabolism Exosomes/metabolism Gene Knockdown Techniques Gene Transfer Techniques Genetic Vectors Lysosomal-Associated Membrane Protein 2/genetics Male Membrane Proteins/metabolism Mice Mice, Inbred C57BL Microglia/metabolism Neurons/metabolism Peptides/metabolism Proteins/metabolism RNA, Messenger/metabolism RNA, Small Interfering/administration & dosage Rabies virus/metabolism
Chemicals
Lysosomal-Associated Membrane Protein 2 Membrane Proteins Peptides Proteins RNA, Messenger RNA, Small Interfering Amyloid Precursor Protein Secretases Aspartic Acid Endopeptidases Bace1 protein, mouse
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Alvarez-Erviti Lydia
Department of Physiology, Anatomy and Genetics, University of Oxford, Oxford, UK.
Seow Yiqi
Yin Haifang
Betts Corinne
Lakhal Samira
Wood Matthew J A
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Article Info
Journal
Nature biotechnology
Abbr.
Nat Biotechnol
ISSN
1546-1696
Published
2011-04-00
Epub
2011-00-20
Pages
341-5
Language
English
Region
United States
NLM ID
9604648
Subset
IM
Grants
Parkinson's UK · G-0904 · United Kingdom
Medical Research Council · G0900887 · United Kingdom
Parkinson's UK · J-0901 · United Kingdom
Corrections
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