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PMID: 23335630 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Dynamics and biological relevance of DNA demethylation in Arabidopsis antibacterial defense.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 110 ·No. 6 ·2013-02-05 ·Pages 2389-94

Yu A, Lepère G, Jay F, Wang J, Bapaume L, Wang Y, Abraham AL, Penterman J, Fischer RL, Voinnet O, Navarro L

Abstract

DNA methylation is an epigenetic mark that silences transposable elements (TEs) and repeats. Whereas the establishment and maintenance of DNA methylation are relatively well understood, little is known about their dynamics and biological relevance in plant and animal innate immunity. Here, we show that some TEs are demethylated and transcriptionally reactivated during antibacterial defense in Arabidopsis. This effect is correlated with the down-regulation of key transcriptional gene silencing factors and is partly dependent on an active demethylation process. DNA demethylation restricts multiplication and vascular propagation of the bacterial pathogen Pseudomonas syringae in leaves and, accordingly, some immune-response genes, containing repeats in their promoter regions, are negatively regulated by DNA methylation. This study provides evidence that DNA demethylation is part of a plant-induced immune response, potentially acting to prime transcriptional activation of some defense genes linked to TEs/repeats.

MeSH Terms
Arabidopsis/genetics,immunology,metabolism,microbiology Arabidopsis Proteins/genetics,metabolism Base Sequence DNA Methylation DNA, Plant/genetics,metabolism Genes, Plant Host-Pathogen Interactions/genetics,immunology Molecular Sequence Data Mutation Plant Diseases/genetics,immunology,microbiology Plant Leaves/immunology,metabolism,microbiology Plants, Genetically Modified Promoter Regions, Genetic Protein Kinases/genetics,metabolism Pseudomonas syringae/immunology,pathogenicity RNA, Small Interfering/genetics
Chemicals
Arabidopsis Proteins DNA, Plant RNA, Small Interfering Protein Kinases FLS2 protein, Arabidopsis
Authors & Affiliations
11 authors, click to expand affiliations / ORCID
Yu Agnès
Centre National de la Recherche Scientifique, Unité Mixte de Recherche 8197, Institut National de la Santé et de la Recherche Médicale, Unité 1024, 75005 Paris, France.
Lepère Gersende
Jay Florence
Wang Jingyu
Bapaume Laure
Wang Yu
Abraham Anne-Laure
Penterman Jon
Fischer Robert L
Voinnet Olivier
Navarro Lionel
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
1091-6490
DOI
10.1073/pnas.1211757110
Published
2013-02-05
Epub
2013-00-18
Pages
2389-94
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC3568381
Subset
IM
Grants
NIGMS NIH HHS · R01 GM069415 · United States
NIGMS NIH HHS · R01GM069415 · United States
Databases
GEO
GSE40044
External Links
PubMed source DOI: 10.1073/pnas.1211757110 PMC Full Text View journal details
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