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PMID: 2332402 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Characterization of Halobacterium halobium mutants defective in taxis.

Journal of bacteriology ·Vol. 172 ·No. 5 ·1990-05-00 ·Pages 2328-35

Sundberg SA, Alam M, Lebert M, Spudich JL, Oesterhelt D, Hazelbauer GL

Abstract

Mutant derivatives of Halobacterium halobium previously isolated by using a procedure that selected for defective phototactic response to white light were examined for an array of phenotypic characteristics related to phototaxis and chemotaxis. The properties tested were unstimulated swimming behavior, behaviorial responses to temporal gradients of light and spatial gradients of chemoattractants, content of photoreceptor pigments, methylation of methyl-accepting taxis proteins, and transient increases in rate of release of volatile methyl groups induced by tactic stimulation. Several distinct phenotypes were identified, corresponding to a mutant missing photoreceptors, a mutant defective in the methyltransferase, a mutant altered in control of the methylesterase, and mutants apparently defective in intracellular signaling. All except the photoreceptor mutant were defective in both chemotaxis and phototaxis.

MeSH Terms
Bacterial Proteins/isolation & purification,metabolism Cell Movement Halobacterium/genetics,physiology Kinetics Light Methionine/metabolism Methylation Mutation Rhodopsin
Chemicals
Bacterial Proteins Rhodopsin Methionine
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Sundberg S A
Cardiovascular Research Institute, University of California, San Francisco 94143.
Alam M
Lebert M
Spudich J L
Oesterhelt D
Hazelbauer G L
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34 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.172.5.2328-2335.1990
Published
1990-05-00
Pages
2328-35
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC208866
Subset
IM
Grants
NIGMS NIH HHS · GM27750 · United States
NIGMS NIH HHS · GM29936 · United States
NIGMS NIH HHS · GM34219 · United States
External Links
PubMed source DOI: 10.1128/jb.172.5.2328-2335.1990 PMC Full Text View journal details
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