Biosynthesis of the two halobacterial light sensors P480 and sensory rhodopsin and variation in gain of their signal transduction chains.

Journal of bacteriology ·Vol. 171 ·No. 4 ·1989-04-00 ·Pages 2155-9

Otomo J, Marwan W, Oesterhelt D, Desel H, Uhl R

Abstract

The two retinal-containing photoreceptors of halobacteria, P480 and sensory rhodopsin, are formed constitutively and inducibly, respectively. Both photoreceptors are synthesized as apoproteins in cells with nicotine-inhibited retinal synthesis and are reconstituted as chromoproteins by the addition of all-trans retinal to cell membrane preparations. The decrease in photoreceptor-mediated photophobic response at the stationary growth phase of cells is not due to photoreceptor degradation but due to a deficiency of the signal transduction chain in the cell.

MeSH Terms

Bacterial Proteins/biosynthesis Bacteriorhodopsins/physiology Cell Membrane/physiology Halobacterium/physiology Light Photoreceptor Cells/physiology Retinaldehyde/metabolism Spectrum Analysis

Chemicals

Bacterial Proteins Bacteriorhodopsins Retinaldehyde

Authors & Affiliations

5 authors, click to expand affiliations / ORCID

Otomo J

Max-Planck-Institut für Biochem e, Martinsried, Federal Republic of Germany.

Marwan W

Oesterhelt D

Desel H

Uhl R

References (15)

15 references, click to expand

Isolation and properties of the native chromoprotein halorhodopsin.
EMBO J. 1983;2(8):1379-85 PMID: 10872334
Single photon detection by an archaebacterium.
J Mol Biol. 1988 Feb 20;199(4):663-4 PMID: 3127593
Two photosystems controlling behavioural responses of Halobacterium halobium.
Nature. 1975 Sep 4;257(5521):46-8 PMID: 1161001
Biosynthesis of the purple membrane of halobacteria.
Angew Chem Int Ed Engl. 1976 Apr;15(4):187-94 PMID: 822754
Light Antennas in phototactic algae.
Microbiol Rev. 1980 Dec;44(4):572-630 PMID: 7010112
Control of transmembrane ion fluxes to select halorhodopsin-deficient and other energy-transduction mutants of Halobacterium halobium.
Proc Natl Acad Sci U S A. 1982 Jul;79(14):4308-12 PMID: 6289299
Identification of a third rhodopsin-like pigment in phototactic Halobacterium halobium.
Proc Natl Acad Sci U S A. 1982 Oct;79(20):6250-4 PMID: 6959114
Two photocycles in halobacterium halobium that lacks bacteriorhodopsin.
Biochem Biophys Res Commun. 1982 Oct 15;108(3):970-6 PMID: 7181896
Mechanism of colour discrimination by a bacterial sensory rhodopsin.
Nature. 1984 Dec 6-12;312(5994):509-13 PMID: 6504161
A polychromatic flash photolysis apparatus (PFPA).
J Biochem Biophys Methods. 1984 Nov;10(1-2):35-48 PMID: 6520324
Selection and properties of phototaxis-deficient mutants of Halobacterium halobium.
J Bacteriol. 1985 Oct;164(1):282-7 PMID: 4044522
Color discrimination in halobacteria: spectroscopic characterization of a second sensory receptor covering the blue-green region of the spectrum.
Proc Natl Acad Sci U S A. 1986 Oct;83(19):7272-6 PMID: 3463965
Flash spectrophotometric identification of a fourth rhodopsin-like pigment in Halobacterium halobium.
Biochem Biophys Res Commun. 1986 Sep 14;139(2):389-95 PMID: 3767969
Signal formation in the halobacterial photophobic response mediated by a fourth retinal protein (P480).
J Mol Biol. 1987 May 20;195(2):333-42 PMID: 3656416
Isolation of the cell membrane of Halobacterium halobium and its fractionation into red and purple membrane.
Methods Enzymol. 1974;31:667-78 PMID: 4418026

Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.171.4.2155-2159.1989

Published

1989-04-00

Pages

2155-9

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC209871

Subset

External Links

PubMed source DOI: 10.1128/jb.171.4.2155-2159.1989 PMC Full Text View journal details

Analysis Services

Biosynthesis of the two halobacterial light sensors P480 and sensory rhodopsin and variation in gain of their signal transduction chains.

Contact

WeChat Official Account