A cleavage map of the TOL plasmid of Pseudomonas putida mt-2.

Molecular & general genetics : MGG ·Vol. 177 ·No. 1 ·1979-00-00 ·Pages 189-91

Downing R, Broda P

Abstract

A cleavage map of the TOL plasmid pWWO has been determined for the restriction endonucleases HindIII and XhoI. A number of techniques were employed including (i) digestion of purified cleavage products with a second enzyme; (ii) hybridisation of purified XhoI fragments to Southern blots of HindIII digest products and (iii) analysis of a number of deletion mutants.

MeSH Terms

Chromosome Mapping Chromosomes, Bacterial/ultrastructure DNA Restriction Enzymes Plasmids Pseudomonas/genetics,metabolism,ultrastructure Toluene/metabolism Xylenes/metabolism

Chemicals

Xylenes Toluene DNA Restriction Enzymes

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Downing R

Broda P

References (8)

8 references, click to expand

Transposition of plasmid DNA segments specifying hydrocarbon degradation and their expression in various microorganisms.
Proc Natl Acad Sci U S A. 1978 Jul;75(7):3109-12 PMID: 277912
Transposition of Pseudomonas toluene-degrading genes and expression in Escherichia coli.
Nature. 1978 Jul 13;274(5667):179-80 PMID: 96356
Two modes of loss of the Tol function from Pseudomonas putida mt-2.
Mol Gen Genet. 1977 Jul 20;154(2):203-4 PMID: 895716
TOL is a broad-host-range plasmid.
J Bacteriol. 1978 Jul;135(1):278-80 PMID: 97271
Restriction endonuclease mapping of a plasmid that confers oncogenicity upon Agrobacterium tumefaciens strain B6-806.
Plasmid. 1978 Feb;1(2):254-69 PMID: 748950
An endonuclease cleavage map of the plasmid pWWO-8, a derivative of the TOL plasmid of Pseudomonas putida mt-2.
Mol Gen Genet. 1979 Jan 5;168(1):97-99 PMID: 285316
DNA-DNA hybridization on membrane filters: a convenient method using formamide.
Biochim Biophys Acta. 1977 Jul 15;477(2):191-4 PMID: 406929
Isolation of TOL and RP4 recombinants by integrative suppression.
J Bacteriol. 1978 Apr;134(1):270-7 PMID: 418059

Article Info

Journal

Molecular & general genetics : MGG

Abbr.

Mol Gen Genet

ISSN

0026-8925

DOI

10.1007/BF00267270

Published

1979-00-00

Pages

189-91

Language

English

Region

Germany

NLM ID

0125036

Subset

External Links

PubMed source DOI: 10.1007/BF00267270

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A cleavage map of the TOL plasmid of Pseudomonas putida mt-2.

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