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PMID: 23169059 Published · ppublish English Journal Article Research Support, N.I.H., Extramural

Supporting conditional mouse mutagenesis with a comprehensive cre characterization resource.

Nature communications ·Vol. 3 ·2012-00-00 ·Pages 1218

Heffner CS, Herbert Pratt C, Babiuk RP, Sharma Y, Rockwood SF, Donahue LR, Eppig JT, Murray SA

Abstract

Full realization of the value of the loxP-flanked alleles generated by the International Knockout Mouse Consortium will require a large set of well-characterized cre-driver lines. However, many cre driver lines display excision activity beyond the intended tissue or cell type, and these data are frequently unavailable to the potential user. Here we describe a high-throughput pipeline to extend characterization of cre driver lines to document excision activity in a wide range of tissues at multiple time points and disseminate these data to the scientific community. Our results show that the majority of cre strains exhibit some degree of unreported recombinase activity. In addition, we observe frequent mosaicism, inconsistent activity and parent-of-origin effects. Together, these results highlight the importance of deep characterization of cre strains, and provide the scientific community with a critical resource for cre strain information.

MeSH Terms
Animals Embryo, Mammalian/metabolism Fatty Acid-Binding Proteins/metabolism Female Genes, Reporter Inheritance Patterns/genetics Integrases/metabolism Male Mice Mutagenesis/genetics Recombination, Genetic/genetics Reproducibility of Results Transgenes/genetics
Chemicals
Fabp4 protein, mouse Fatty Acid-Binding Proteins Cre recombinase Integrases
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Heffner Caleb S
The Jackson Laboratory, 600 Main Street, Bar Harbor, Maine 04609, USA.
Herbert Pratt C
Babiuk Randal P
Sharma Yashoda
Rockwood Stephen F
Donahue Leah R
Eppig Janan T
Murray Stephen A
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Article Info
Journal
Nature communications
Abbr.
Nat Commun
ISSN
2041-1723
DOI
10.1038/ncomms2186
Published
2012-00-00
Pages
1218
Language
English
Region
England
NLM ID
101528555
PMCID
PMC3514490
Subset
IM
Grants
NHGRI NIH HHS · HG000330 · United States
NCRR NIH HHS · R21 RR026117 · United States
NIH HHS · R24 OD011190 · United States
NHGRI NIH HHS · U41 HG000330 · United States
NCRR NIH HHS · RR026117 · United States
NCI NIH HHS · CA034196 · United States
NIH HHS · OD011190 · United States
NHGRI NIH HHS · P41 HG000330 · United States
NCI NIH HHS · P30 CA034196 · United States
External Links
PubMed source DOI: 10.1038/ncomms2186 PMC Full Text View journal details
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