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PMID: 23049605 Published · ppublish English Journal Article

The Antitumor Activity of Antrodia camphorata in Melanoma Cells: Modulation of Wnt/β-Catenin Signaling Pathways.

Evidence-based complementary and alternative medicine : eCAM ·Vol. 2012 ·2012-00-00 ·Pages 197309

Hseu YC, Tsou HT, Kumar KJ, Lin KY, Chang HW, Yang HL

Abstract

Antrodia camphorata (AC) is well known in Taiwan as a traditional Chinese medicine. The aim of this study was to investigate whether a fermented culture broth of AC could inhibit melanoma proliferation and progression via suppression of the Wnt/β-catenin signaling pathway. In this study, we observed that AC treatment resulted in decreased cell viability and disturbed Wnt/β-catenin cascade in B16F10 and/or B16F1 melanoma cells. This result was accompanied by a decrease in the expression of Wnt/β-catenin transcriptional targets, including c-Myc and survivin. Furthermore, treatment of melanoma cells with AC resulted in a significant increase in apoptosis, which was associated with DNA fragmentation, cytochrome c release, caspase-9 and -3 activation, PARP degradation, Bcl-2/Bax dysregulation, and p53 expression. We also observed that AC caused G(1) phase arrest mediated by a downregulation of cyclin D1 and CDK4 and increased p21 and p27 expression. In addition, we demonstrated that non- and subcytotoxic concentrations of AC markedly inhibited migration and invasion of highly metastatic B16F10 cells. The antimetastatic effect of AC was further confirmed by reductions in the levels of MMP-2, MMP-9, and VEGF expression. These results suggest that Antrodia camphorata may exert antitumor activity by downregulating the Wnt/β-catenin pathways.

Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Hseu You-Cheng
Department of Cosmeceutics, College of Pharmacy, China Medical University, Taichung 40402, Taiwan.
Tsou Hsiao-Tung
Kumar K J Senthil
Lin Kai-Yuan
Chang Hsueh-Wei
Yang Hsin-Ling
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Article Info
Journal
Evidence-based complementary and alternative medicine : eCAM
Abbr.
Evid Based Complement Alternat Med
ISSN
1741-4288
DOI
10.1155/2012/197309
Published
2012-00-00
Epub
2012-00-25
Pages
197309
Language
English
Region
United States
NLM ID
101215021
PMCID
PMC3463817
External Links
PubMed source DOI: 10.1155/2012/197309 PMC Full Text
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