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PMID: 2300586 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

The 5HT2 receptor defines a family of structurally distinct but functionally conserved serotonin receptors.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 87 ·No. 3 ·1990-02-00 ·Pages 928-32

Julius D, Huang KN, Livelli TJ, Axel R, Jessell TM

Abstract

Serotonin exerts its diverse physiological effects by interacting with multiple distinct receptor subtypes. We have isolated a rat brain 5HT2 serotonin receptor cDNA by virtue of its homology with the 5HT1c receptor. The 5HT2 receptor is a member of the family of receptors that are linked to guanine nucleotide-binding proteins and are predicted to span the lipid bilayer seven times. Overall sequence identity between the 5HT2 and 5HT1c receptors is 49%, but identity within the transmembrane domains is 80%. Expression of both the 5HT2 and 5HT1c receptors in transfected mouse fibroblasts activates phospholipase C signaling pathways and promotes cellular transformation. However, RNA blotting shows that these two receptor subtypes are differentially expressed in the central nervous system. In this manner, structurally and functionally homologous receptor subtypes may elicit distinct physiologic actions.

MeSH Terms
Amino Acid Sequence Animals Antiparkinson Agents/pharmacology Brain/metabolism DNA/genetics,isolation & purification Ergolines/pharmacology Fibroblasts/metabolism Gene Library Genes Mice Molecular Sequence Data Organ Specificity RNA, Messenger/analysis,genetics Rats Receptors, Serotonin/drug effects,genetics,metabolism Sequence Homology, Nucleic Acid Spiperone/pharmacology Transfection Type C Phospholipases/metabolism
Chemicals
Antiparkinson Agents Ergolines RNA, Messenger Receptors, Serotonin Spiperone DNA Type C Phospholipases mesulergine
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Julius D
Department of Biochemistry and Molecular Biophysics, College of Physicians and Surgeons, Columbia University, New York, NY 10032.
Huang K N
Livelli T J
Axel R
Jessell T M
References (27)
27 references, click to expand
  1. Identification of a family of muscarinic acetylcholine receptor genes.
    Science. 1987 Jul 31;237(4814):527-32 PMID: 3037705
  2. Molecular cloning and primary structure of myelin-associated glycoprotein.
    Proc Natl Acad Sci U S A. 1987 Jan;84(2):600-4 PMID: 2432614
  3. An intronless gene encoding a potential member of the family of receptors coupled to guanine nucleotide regulatory proteins.
    Nature. 1987 Sep 3-9;329(6134):75-9 PMID: 3041227
  4. Distinct primary structures, ligand-binding properties and tissue-specific expression of four human muscarinic acetylcholine receptors.
    EMBO J. 1987 Dec 20;6(13):3923-9 PMID: 3443095
  5. Construction and characterization of a retroviral vector demonstrating efficient expression of cloned cDNA sequences.
    DNA. 1988 Apr;7(3):219-25 PMID: 2836147
  6. 5-HT1D receptor-mediated inhibition of forskolin-stimulated adenylate cyclase activity in calf substantia nigra.
    Eur J Pharmacol. 1988 Feb 16;147(1):145-7 PMID: 3371410
  7. The coupling of neurotransmitter receptors to ion channels in the brain.
    Science. 1988 Jul 29;241(4865):545-51 PMID: 2456612
  8. Molecular characterization of a functional cDNA encoding the serotonin 1c receptor.
    Science. 1988 Jul 29;241(4865):558-64 PMID: 3399891
  9. The genomic clone G-21 which resembles a beta-adrenergic receptor sequence encodes the 5-HT1A receptor.
    Nature. 1988 Sep 22;335(6188):358-60 PMID: 3138543
  10. Flow cytometric identification and purification of cells by ligand-induced changes in intracellular calcium.
    J Neurosci Methods. 1988 Nov;26(1):35-44 PMID: 2462137
  11. Structure and functional expression of cloned rat serotonin 5HT-2 receptor.
    EMBO J. 1988 Dec 20;7(13):4135-40 PMID: 2854054
  12. Ectopic expression of the serotonin 1c receptor and the triggering of malignant transformation.
    Science. 1989 Jun 2;244(4908):1057-62 PMID: 2727693
  13. Functional role of muscarinic acetylcholine receptor subtype diversity.
    Cold Spring Harb Symp Quant Biol. 1988;53 Pt 1:263-72 PMID: 3254774
  14. Functional expression of the 5-HT1c receptor in neuronal and nonneuronal cells.
    Cold Spring Harb Symp Quant Biol. 1988;53 Pt 1:385-93 PMID: 3254776
  15. 5-HT1c receptor is a prominent serotonin receptor subtype in the central nervous system.
    Proc Natl Acad Sci U S A. 1989 Sep;86(17):6793-7 PMID: 2771958
  16. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  17. The arrangement and rearrangement of antibody genes.
    Nature. 1978 Dec 21-28;276(5690):790-5 PMID: 103004
  18. The rat serum albumin gene: analysis of cloned sequences.
    Proc Natl Acad Sci U S A. 1979 Jul;76(7):3256-60 PMID: 158758
  19. 2-[125Iodo]LSD, a new ligand for the characterisation and localisation of 5-HT2 receptors.
    Naunyn Schmiedebergs Arch Pharmacol. 1984 Apr;325(4):328-36 PMID: 6728042
  20. A new generation of Ca2+ indicators with greatly improved fluorescence properties.
    J Biol Chem. 1985 Mar 25;260(6):3440-50 PMID: 3838314
  21. Evidence that phospholipid turnover is the signal transducing system coupled to serotonin-S2 receptor sites.
    J Biol Chem. 1985 Jun 25;260(12):7603-8 PMID: 3922987
  22. Quantitative autoradiographic mapping of serotonin receptors in the rat brain. I. Serotonin-1 receptors.
    Brain Res. 1985 Nov 4;346(2):205-30 PMID: 4052776
  23. Serotonin 5-HT1 receptors mediate inhibition of cyclic AMP production in neurons.
    Eur J Pharmacol. 1986 Jan 21;120(2):227-30 PMID: 3005002
  24. Cloning of the gene and cDNA for mammalian beta-adrenergic receptor and homology with rhodopsin.
    Nature. 1986 May 1-7;321(6065):75-9 PMID: 3010132
  25. A unique serotonin receptor in choroid plexus is linked to phosphatidylinositol turnover.
    Proc Natl Acad Sci U S A. 1986 Jun;83(11):4086-8 PMID: 2940597
  26. Characterization of the 5-hydroxytryptamine1a receptor-mediated inhibition of forskolin-stimulated adenylate cyclase activity in guinea pig and rat hippocampal membranes.
    J Pharmacol Exp Ther. 1986 Jul;238(1):248-53 PMID: 2941565
  27. A family of receptors coupled to guanine nucleotide regulatory proteins.
    Biochemistry. 1987 May 19;26(10):2657-64 PMID: 3038163
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.87.3.928
Published
1990-02-00
Pages
928-32
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC53382
Subset
IM
Databases
GENBANK
M30705
External Links
PubMed source DOI: 10.1073/pnas.87.3.928 PMC Full Text View journal details
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