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PMID: 22960039 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

The dREAM/Myb-MuvB complex and Grim are key regulators of the programmed death of neural precursor cells at the Drosophila posterior wing margin.

Developmental biology ·Vol. 372 ·No. 1 ·2012-12-01 ·Pages 88-102

Rovani MK, Brachmann CB, Ramsay G, Katzen AL

Abstract

Successful development of a multicellular organism depends on the finely tuned orchestration of cell proliferation, differentiation and apoptosis from embryogenesis through adulthood. The MYB-gene family encodes sequence-specific DNA-binding transcription factors that have been implicated in the regulation of both normal and neoplastic growth. The Drosophila Myb protein, DMyb (and vertebrate B-Myb protein), has been shown to be part of the dREAM/MMB complex, a large multi-subunit complex, which in addition to four Myb-interacting proteins including Mip130, contains repressive E2F and pRB proteins. This complex has been implicated in the regulation of DNA replication within the context of chorion gene amplification and transcriptional regulation of a wide array of genes. Detailed phenotypic analysis of mutations in the Drosophila myb gene, Dm myb, has revealed a previously undiscovered function for the dREAM/MMB complex in regulating programmed cell death (PCD). In cooperation with the pro-apoptotic protein Grim and dREAM/MMB, DMyb promotes the PCD of specified sensory organ precursor daughter cells in at least two different settings in the peripheral nervous system: the pIIIb precursor of the neuron and sheath cells in the posterior wing margin and the glial cell in the thoracic microchaete lineage. Unlike previously analyzed settings, in which the main role of DMyb has been to antagonize the activities of other dREAM/MMB complex members, it appears to be the critical effector in promoting PCD. The finding that Dm myb and grim are both involved in regulating PCD in two distinct settings suggests that these two genes may often work together to mediate PCD.

MeSH Terms
Animals Apoptosis Carrier Proteins/genetics,metabolism Caspases/genetics,metabolism Cell Cycle Proteins/genetics,metabolism Cell Death Cell Differentiation Cell Lineage Cell Proliferation DNA Replication Drosophila/embryology,genetics,metabolism Drosophila Proteins/genetics,metabolism E2F Transcription Factors/genetics,metabolism Genes, myb Neurons/metabolism Neuropeptides/genetics,metabolism Proto-Oncogene Proteins c-myb/genetics,metabolism Transcription Factors Wings, Animal/embryology,metabolism
Chemicals
Carrier Proteins Cell Cycle Proteins Drosophila Proteins E2F Transcription Factors E2f1 protein, Drosophila Mip130 protein, Drosophila Myb protein, Drosophila Neuropeptides Proto-Oncogene Proteins c-myb Transcription Factors grim protein, Drosophila Caspases STRICA protein, Drosophila
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Rovani Margritte K
Department of Biochemistry and Molecular Genetics University of Illinois at Chicago, 900 South Ashland Avenue, Chicago, IL 60607-7170, USA.
Brachmann Carrie Baker
Ramsay Gary
Katzen Alisa L
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Article Info
Journal
Developmental biology
Abbr.
Dev Biol
ISSN
1095-564X
DOI
10.1016/j.ydbio.2012.08.022
Published
2012-12-01
Epub
2012-00-29
Pages
88-102
Language
English
Region
United States
NLM ID
0372762
PMCID
PMC3621911
Subset
IM
Grants
NIGMS NIH HHS · R01 GM068961 · United States
NIGMS NIH HHS · GM68961 · United States
External Links
PubMed source DOI: 10.1016/j.ydbio.2012.08.022 PMC Full Text View journal details
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