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PMID: 229147 Published · ppublish English Comparative Study Journal Article

Prolactin receptors: a comparison between chloramine-T and lactoperoxidase iodination.

Journal of endocrinological investigation ·Vol. 1 ·No. 3 ·1978-07-00 ·Pages 233-8

Kuo-Jang K, Ramirez VD

Abstract

The binding properties of chloramine-T iodinated oPRL (using a modified iodination procedure) to prolactin receptors of female rat liver membrane particles were compared with those of lactoperoxidase iodinated oPRL. The results indicated that both iodination methods provided 125I-oPRLs which were suitable for receptor binding studies. Our results suggested that chloramine-T 125I-oPRL was even better than lactoperoxidase 125I-oPRL in terms of lower nonspecific binding. The chloramine-T iodinated oPRL was used to study the prolactin receptors in rat ovaries and DMBA induced rat mammary tumors. The results showed that the amount of prolactin receptors in rat ovaries was related to the plasma level of prolactin as it had been reported in liver by other investigators. The study of prolactin receptors in rat mammary tumors indicated that the prolactin receptor content of hormone dependent mammary tumors was much higher than that of hormone independent mammary tumors.

MeSH Terms
Animals Binding, Competitive Chloramines/pharmacology Dose-Response Relationship, Drug Female Iodine Radioisotopes Isotope Labeling/methods Lactoperoxidase/pharmacology Liver/analysis Mammary Neoplasms, Experimental/analysis Ovary/analysis Peroxidases/pharmacology Prolactin/blood,physiology Rats Receptors, Cell Surface/analysis
Chemicals
Chloramines Iodine Radioisotopes Receptors, Cell Surface Prolactin Lactoperoxidase Peroxidases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Kuo-Jang K
Ramirez V D
References (18)
18 references, click to expand
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Article Info
Journal
Journal of endocrinological investigation
Abbr.
J Endocrinol Invest
ISSN
0391-4097
DOI
10.1007/BF03350386
Published
1978-07-00
Pages
233-8
Language
English
Region
Italy
NLM ID
7806594
Subset
IM
External Links
PubMed source DOI: 10.1007/BF03350386 View journal details
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